A deletion in the sapA homologue cluster is responsible for the loss of the S-layer in Campylobacter fetus strain TK

Masaki Fujita, Tetsuhiro Moriya, Shuji Fujimoto, Nobuyuki Hara, Kazunobu Amako

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The surface array protein (SAP) of Campylobacter fetus strain TK is encoded by seven homologous sapA genes clustered on the chromosomal DNA. The spontaneously arising variant strain TK(SAP-) produces no SAP and carries an approximately 10-kb chromosomal deletion. To elucidate the mechanism underlying the loss of SAP synthesis, we analyzed the region containing the sapA homologues and the deletion. We constructed a physical map of the sapA cluster region by aligning the clones that contain sapA homologues. These analyses demonstrated that all sapA homologues were located within a limited region of about 50 kb of chromosomal DNA of strain TK. The TK(SAP-) deletion was located within this cluster and was 13.3 kb in size. The deletion occurred between two sapA homologues and resulted in the formation of a chimeric sapA homologue in the variant strain. Sequence analysis of the upstream regions and the conserved regions of all sapA homologues revealed a high degree of similarity. However, only one sapA homologue contained a putative promoter sequence. This promoter sequence was located in the deleted region. Thus, the deletion of the promoter appears to be responsible for the loss of SAP expression in TK(SAP-).

Original languageEnglish
Pages (from-to)196-201
Number of pages6
JournalArchives of Microbiology
Volume167
Issue number4
DOIs
Publication statusPublished - May 21 1997

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Campylobacter fetus
DNA
S-layer proteins
Sequence Analysis
Clone Cells
Genes

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Biochemistry
  • Molecular Biology
  • Genetics

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A deletion in the sapA homologue cluster is responsible for the loss of the S-layer in Campylobacter fetus strain TK. / Fujita, Masaki; Moriya, Tetsuhiro; Fujimoto, Shuji; Hara, Nobuyuki; Amako, Kazunobu.

In: Archives of Microbiology, Vol. 167, No. 4, 21.05.1997, p. 196-201.

Research output: Contribution to journalArticle

Fujita, Masaki ; Moriya, Tetsuhiro ; Fujimoto, Shuji ; Hara, Nobuyuki ; Amako, Kazunobu. / A deletion in the sapA homologue cluster is responsible for the loss of the S-layer in Campylobacter fetus strain TK. In: Archives of Microbiology. 1997 ; Vol. 167, No. 4. pp. 196-201.
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AU - Amako, Kazunobu

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