A DNA hybridization system for labeling of neural stem cells with SPIO nanoparticles for MRI monitoring post-transplantation

Edgar Y. Egawa, Narufumi Kitamura, Ryusuke Nakai, Yusuke Arima, Hiroo Iwata

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Neural stem cells (NSCs) demonstrate encouraging results in cell replacement therapy for neurodegenerative disorders and traumatic injury in the central nervous system. Monitor the survival and migration of transplanted cells would provide us important information concerning the performance and integration of the graft during the therapy time course. Magnetic resonance imaging (MRI) allow us to monitor the transplanted cells in a non-invasive way. The only requirement is to use an appropriate contrast agent to label the transplanted cells. Superparamagnetic iron oxide (SPIO) nanoparticles are one of the most commonly used contrast agent for MRI detection of transplanted cells. SPIO nanoparticles demonstrated to be suitable for labeling several types of cells including NSCs. However, the current methods for SPIO labeling are non-specific, depending mostly on electrostatic interactions, demanding relatively high SPIO concentration, and long incubation time, which can affect the viability of cells. In this study, we propose a specific and relatively fast method to label NSCs with SPIO nanoparticles via DNA hybridization. Two short single stranded DNAs (ssDNAs), oligo[dT]20 and oligo[dA]20 were conjugated with a lipid molecule and SPIO nanoparticle respectively. The labeling process comprises two simple steps; first the cells are modified to present oligo[dT]20 ssDNA on the cell surface, then the oligo[dA]20 ssDNA conjugated with SPIO nanoparticles are presented to the modified cells to allow the oligo[dT]20-oligo[dA]20 hybridization. The method showed to be non-toxic at concentrations up to 50μg/mL oligo[dA]20-SPIO nanoparticles. Presence of SPIO nanoparticles at cell surface and cell cytoplasm was verified by transmission electron microscopy (TEM). SPIO labeling via DNA hybridization demonstrated to not interfere on NSCs proliferation, aggregates formation, and differentiation. NSCs labeled with SPIO nanoparticles via DNA hybridization system were successfully detected by MRI invitro as well invivo. Cells transplanted into the rat brain striatum could be detected by MRI scanning up to 1 month post-transplantation.

Original languageEnglish
Pages (from-to)158-167
Number of pages10
JournalBiomaterials
Volume54
DOIs
Publication statusPublished - Jun 1 2015
Externally publishedYes

Fingerprint

Neural Stem Cells
Stem cells
Iron oxides
Nanoparticles
Magnetic resonance imaging
Labeling
DNA
Transplantation
Magnetic Resonance Imaging
Monitoring
Contrast Media
Labels
ferric oxide
Single-Stranded DNA
Cell proliferation
Neurology
Cell- and Tissue-Based Therapy
Coulomb interactions
Static Electricity
Transmission Electron Microscopy

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Ceramics and Composites
  • Biophysics
  • Biomaterials
  • Mechanics of Materials

Cite this

A DNA hybridization system for labeling of neural stem cells with SPIO nanoparticles for MRI monitoring post-transplantation. / Egawa, Edgar Y.; Kitamura, Narufumi; Nakai, Ryusuke; Arima, Yusuke; Iwata, Hiroo.

In: Biomaterials, Vol. 54, 01.06.2015, p. 158-167.

Research output: Contribution to journalArticle

Egawa, Edgar Y. ; Kitamura, Narufumi ; Nakai, Ryusuke ; Arima, Yusuke ; Iwata, Hiroo. / A DNA hybridization system for labeling of neural stem cells with SPIO nanoparticles for MRI monitoring post-transplantation. In: Biomaterials. 2015 ; Vol. 54. pp. 158-167.
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