A DNA size probe based on energy migration in crosslinked chromatin

Maged F. Serag; Noritada Kaji; Yukihiro Okamoto; Manabu Tokeshi; Yoshinobu Baba

A DNA size probe based on energy migration in crosslinked chromatin

Maged F. Serag, Noritada Kaji, Yukihiro Okamoto, Manabu Tokeshi, Yoshinobu Baba

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

DNA sizing has a central role in virtually every aspect of physical genomic analysis. Although capillary gel electrophoresis adequately separates DNA samples, accurate sizing of short sequences is problematic. Here we introduce a simple methodology that affords sizing of short DNA sequences at 2nt resolution via wrapping and linking histone subunits with fluorophore-tagged ssDNA to extract a wrapping and linking ratio that is unique to the reported DNA size.

Original language	English
Title of host publication	15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Pages	58-60
Number of pages	3
Volume	1
Publication status	Published - Dec 1 2011
Externally published	Yes
Event	15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 - Seattle, WA, United States Duration: Oct 2 2011 → Oct 6 2011

Other

Other	15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Country/Territory	United States
City	Seattle, WA
Period	10/2/11 → 10/6/11

All Science Journal Classification (ASJC) codes

Control and Systems Engineering

Cite this

Serag, MF, Kaji, N, Okamoto, Y, Tokeshi, M & Baba, Y 2011, A DNA size probe based on energy migration in crosslinked chromatin. in 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011. vol. 1, pp. 58-60, 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011, Seattle, WA, United States, 10/2/11.

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author = "Serag, {Maged F.} and Noritada Kaji and Yukihiro Okamoto and Manabu Tokeshi and Yoshinobu Baba",

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AU - Serag, Maged F.

AU - Kaji, Noritada

AU - Okamoto, Yukihiro

AU - Tokeshi, Manabu

AU - Baba, Yoshinobu

PY - 2011/12/1

Y1 - 2011/12/1

N2 - DNA sizing has a central role in virtually every aspect of physical genomic analysis. Although capillary gel electrophoresis adequately separates DNA samples, accurate sizing of short sequences is problematic. Here we introduce a simple methodology that affords sizing of short DNA sequences at 2nt resolution via wrapping and linking histone subunits with fluorophore-tagged ssDNA to extract a wrapping and linking ratio that is unique to the reported DNA size.

AB - DNA sizing has a central role in virtually every aspect of physical genomic analysis. Although capillary gel electrophoresis adequately separates DNA samples, accurate sizing of short sequences is problematic. Here we introduce a simple methodology that affords sizing of short DNA sequences at 2nt resolution via wrapping and linking histone subunits with fluorophore-tagged ssDNA to extract a wrapping and linking ratio that is unique to the reported DNA size.

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M3 - Conference contribution

AN - SCOPUS:84883828551

SN - 9781618395955

VL - 1

SP - 58

EP - 60

BT - 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011

T2 - 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011

Y2 - 2 October 2011 through 6 October 2011

ER -

A DNA size probe based on energy migration in crosslinked chromatin

Abstract

Other

All Science Journal Classification (ASJC) codes

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