A DNA size probe based on energy migration in crosslinked chromatin

Maged F. Serag; Noritada Kaji; Yukihiro Okamoto; Manabu Tokeshi; Yoshinobu Baba

A DNA size probe based on energy migration in crosslinked chromatin

Maged F. Serag, Noritada Kaji, Yukihiro Okamoto, Manabu Tokeshi, Yoshinobu Baba

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

DNA sizing has a central role in virtually every aspect of physical genomic analysis. Although capillary gel electrophoresis adequately separates DNA samples, accurate sizing of short sequences is problematic. Here we introduce a simple methodology that affords sizing of short DNA sequences at 2nt resolution via wrapping and linking histone subunits with fluorophore-tagged ssDNA to extract a wrapping and linking ratio that is unique to the reported DNA size.

Original language	English
Title of host publication	15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Pages	58-60
Number of pages	3
Volume	1
Publication status	Published - Dec 1 2011
Externally published	Yes
Event	15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 - Seattle, WA, United States Duration: Oct 2 2011 → Oct 6 2011

Other

Other	15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Country	United States
City	Seattle, WA
Period	10/2/11 → 10/6/11

All Science Journal Classification (ASJC) codes

Control and Systems Engineering

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A DNA size probe based on energy migration in crosslinked chromatin. / Serag, Maged F.; Kaji, Noritada; Okamoto, Yukihiro; Tokeshi, Manabu; Baba, Yoshinobu.

15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011. Vol. 1 2011. p. 58-60.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Serag, MF, Kaji, N, Okamoto, Y, Tokeshi, M & Baba, Y 2011, A DNA size probe based on energy migration in crosslinked chromatin. in 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011. vol. 1, pp. 58-60, 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011, Seattle, WA, United States, 10/2/11.

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