A DNA size probe based on energy migration in crosslinked chromatin

Maged F. Serag, Noritada Kaji, Yukihiro Okamoto, Manabu Tokeshi, Yoshinobu Baba

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

DNA sizing has a central role in virtually every aspect of physical genomic analysis. Although capillary gel electrophoresis adequately separates DNA samples, accurate sizing of short sequences is problematic. Here we introduce a simple methodology that affords sizing of short DNA sequences at 2nt resolution via wrapping and linking histone subunits with fluorophore-tagged ssDNA to extract a wrapping and linking ratio that is unique to the reported DNA size.

Original languageEnglish
Title of host publication15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Pages58-60
Number of pages3
Volume1
Publication statusPublished - Dec 1 2011
Externally publishedYes
Event15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 - Seattle, WA, United States
Duration: Oct 2 2011Oct 6 2011

Other

Other15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
CountryUnited States
CitySeattle, WA
Period10/2/1110/6/11

Fingerprint

DNA
Fluorophores
DNA sequences
Electrophoresis
Gels

All Science Journal Classification (ASJC) codes

  • Control and Systems Engineering

Cite this

Serag, M. F., Kaji, N., Okamoto, Y., Tokeshi, M., & Baba, Y. (2011). A DNA size probe based on energy migration in crosslinked chromatin. In 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 (Vol. 1, pp. 58-60)

A DNA size probe based on energy migration in crosslinked chromatin. / Serag, Maged F.; Kaji, Noritada; Okamoto, Yukihiro; Tokeshi, Manabu; Baba, Yoshinobu.

15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011. Vol. 1 2011. p. 58-60.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Serag, MF, Kaji, N, Okamoto, Y, Tokeshi, M & Baba, Y 2011, A DNA size probe based on energy migration in crosslinked chromatin. in 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011. vol. 1, pp. 58-60, 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011, Seattle, WA, United States, 10/2/11.
Serag MF, Kaji N, Okamoto Y, Tokeshi M, Baba Y. A DNA size probe based on energy migration in crosslinked chromatin. In 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011. Vol. 1. 2011. p. 58-60
Serag, Maged F. ; Kaji, Noritada ; Okamoto, Yukihiro ; Tokeshi, Manabu ; Baba, Yoshinobu. / A DNA size probe based on energy migration in crosslinked chromatin. 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011. Vol. 1 2011. pp. 58-60
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