Abstract
DNA sizing has a central role in virtually every aspect of physical genomic analysis. Although capillary gel electrophoresis adequately separates DNA samples, accurate sizing of short sequences is problematic. Here we introduce a simple methodology that affords sizing of short DNA sequences at 2nt resolution via wrapping and linking histone subunits with fluorophore-tagged ssDNA to extract a wrapping and linking ratio that is unique to the reported DNA size.
| Original language | English |
|---|---|
| Title of host publication | 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 |
| Pages | 58-60 |
| Number of pages | 3 |
| Volume | 1 |
| Publication status | Published - Dec 1 2011 |
| Externally published | Yes |
| Event | 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 - Seattle, WA, United States Duration: Oct 2 2011 → Oct 6 2011 |
Other
| Other | 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 |
|---|---|
| Country | United States |
| City | Seattle, WA |
| Period | 10/2/11 → 10/6/11 |
Fingerprint
All Science Journal Classification (ASJC) codes
- Control and Systems Engineering
Cite this
A DNA size probe based on energy migration in crosslinked chromatin. / Serag, Maged F.; Kaji, Noritada; Okamoto, Yukihiro; Tokeshi, Manabu; Baba, Yoshinobu.
15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011. Vol. 1 2011. p. 58-60.Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
}
TY - GEN
T1 - A DNA size probe based on energy migration in crosslinked chromatin
AU - Serag, Maged F.
AU - Kaji, Noritada
AU - Okamoto, Yukihiro
AU - Tokeshi, Manabu
AU - Baba, Yoshinobu
PY - 2011/12/1
Y1 - 2011/12/1
N2 - DNA sizing has a central role in virtually every aspect of physical genomic analysis. Although capillary gel electrophoresis adequately separates DNA samples, accurate sizing of short sequences is problematic. Here we introduce a simple methodology that affords sizing of short DNA sequences at 2nt resolution via wrapping and linking histone subunits with fluorophore-tagged ssDNA to extract a wrapping and linking ratio that is unique to the reported DNA size.
AB - DNA sizing has a central role in virtually every aspect of physical genomic analysis. Although capillary gel electrophoresis adequately separates DNA samples, accurate sizing of short sequences is problematic. Here we introduce a simple methodology that affords sizing of short DNA sequences at 2nt resolution via wrapping and linking histone subunits with fluorophore-tagged ssDNA to extract a wrapping and linking ratio that is unique to the reported DNA size.
UR - http://www.scopus.com/inward/record.url?scp=84883828551&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84883828551&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:84883828551
SN - 9781618395955
VL - 1
SP - 58
EP - 60
BT - 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
ER -