Abstract
DNA sizing has a central role in virtually every aspect of physical genomic analysis. Although capillary gel electrophoresis adequately separates DNA samples, accurate sizing of short sequences is problematic. Here we introduce a simple methodology that affords sizing of short DNA sequences at 2nt resolution via wrapping and linking histone subunits with fluorophore-tagged ssDNA to extract a wrapping and linking ratio that is unique to the reported DNA size.
Original language | English |
---|---|
Title of host publication | 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 |
Pages | 58-60 |
Number of pages | 3 |
Volume | 1 |
Publication status | Published - Dec 1 2011 |
Externally published | Yes |
Event | 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 - Seattle, WA, United States Duration: Oct 2 2011 → Oct 6 2011 |
Other
Other | 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 |
---|---|
Country/Territory | United States |
City | Seattle, WA |
Period | 10/2/11 → 10/6/11 |
All Science Journal Classification (ASJC) codes
- Control and Systems Engineering