A dof transcription factor, SCAP1, is essential for the development of functional stomata in arabidopsis

Juntaro Negi, Kosuke Moriwaki, Mineko Konishi, Ryusuke Yokoyama, Toshiaki Nakano, Kensuke Kusumi, Mimi Hashimoto-Sugimoto, Julian I. Schroeder, Kazuhiko Nishitani, Shuichi Yanagisawa, Koh Iba

Research output: Contribution to journalArticle

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Abstract

Stomata are highly specialized organs that consist of pairs of guard cells and regulate gas and water vapor exchange in plants [1-3]. Although early stages of guard cell differentiation have been described [4-10] and were interpreted in analogy to processes of cell type differentiation in animals [11], the downstream development of functional stomatal guard cells remains poorly understood. We have isolated an Arabidopsis mutant, stomatal carpenter 1 (scap1), that develops irregularly shaped guard cells and lacks the ability to control stomatal aperture, including CO2-induced stomatal closing and light-induced stomatal opening. SCAP1 was identified as a plant-specific Dof-type transcription factor expressed in maturing guard cells, but not in guard mother cells. SCAP1 regulates the expression of genes encoding key elements of stomatal functioning and morphogenesis, such as K+ channel protein, MYB60 transcription factor, and pectin methylesterase. Consequently, ion homeostasis was disturbed in scap1 guard cells, and esterification of extracellular pectins was impaired so that the cell walls lining the pores did not mature normally. We conclude that SCAP1 regulates essential processes of stomatal guard cell maturation and functions as a key transcription factor regulating the final stages of guard cell differentiation.

Original languageEnglish
Pages (from-to)479-484
Number of pages6
JournalCurrent Biology
Volume23
Issue number6
DOIs
Publication statusPublished - Mar 18 2013

Fingerprint

guard cells
Arabidopsis
stomata
Transcription Factors
transcription factors
Pectins
Cell Differentiation
Gene encoding
Plant shutdowns
Steam
Esterification
Linings
Animals
Gases
Cells
cell differentiation
Ions
Morphogenesis
Cell Wall
pectinesterase

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

A dof transcription factor, SCAP1, is essential for the development of functional stomata in arabidopsis. / Negi, Juntaro; Moriwaki, Kosuke; Konishi, Mineko; Yokoyama, Ryusuke; Nakano, Toshiaki; Kusumi, Kensuke; Hashimoto-Sugimoto, Mimi; Schroeder, Julian I.; Nishitani, Kazuhiko; Yanagisawa, Shuichi; Iba, Koh.

In: Current Biology, Vol. 23, No. 6, 18.03.2013, p. 479-484.

Research output: Contribution to journalArticle

Negi, J, Moriwaki, K, Konishi, M, Yokoyama, R, Nakano, T, Kusumi, K, Hashimoto-Sugimoto, M, Schroeder, JI, Nishitani, K, Yanagisawa, S & Iba, K 2013, 'A dof transcription factor, SCAP1, is essential for the development of functional stomata in arabidopsis', Current Biology, vol. 23, no. 6, pp. 479-484. https://doi.org/10.1016/j.cub.2013.02.001
Negi, Juntaro ; Moriwaki, Kosuke ; Konishi, Mineko ; Yokoyama, Ryusuke ; Nakano, Toshiaki ; Kusumi, Kensuke ; Hashimoto-Sugimoto, Mimi ; Schroeder, Julian I. ; Nishitani, Kazuhiko ; Yanagisawa, Shuichi ; Iba, Koh. / A dof transcription factor, SCAP1, is essential for the development of functional stomata in arabidopsis. In: Current Biology. 2013 ; Vol. 23, No. 6. pp. 479-484.
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