A double filtering method for measuring the translational velocity of fluorescently stained cells

Toshiki Yasokawa, Ichirou Ishimaru, Shigeki Kuriyama, Tsutomu Masaki, Kaoru Takegawa, Naotaka Tanaka

Research output: Contribution to journalArticlepeer-review

Abstract

The authors propose a double filtering method to measure translational velocity for tracking fluorescently stained cells. This method employs two diffraction gratings installed in the infinity space through which the parallel pencil beam of the fluorescence passes. With this method, the change in light intensity whose period is proportional to the translational velocity of the sample can be obtained at the imaging surface. By using a sample that has a random distribution of fluorescence intensity, the authors verified that translational velocity measurements could be achieved using the proposed method.

Original languageEnglish
Article number131116
JournalApplied Physics Letters
Volume91
Issue number13
DOIs
Publication statusPublished - Oct 5 2007
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Physics and Astronomy (miscellaneous)

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