Abstract
Therapeutic peptides and diagnostic agents with their molecular size below the renal clearance threshold suffer from short blood circulation time. Here, we report a novel design of peptide-based ligand with a strong binding affinity to human serum albumin (HSA), which can be used as a tag to extend the blood circulation of small-size molecules. We designed ligands with dual alkyl groups connected with a negatively charged spacer. The ligands showed both higher binding affinity to HSA and a higher retention in mice blood than that of a single alkylated peptide.
| Original language | English |
|---|---|
| Pages (from-to) | 501-504 |
| Number of pages | 4 |
| Journal | analytical sciences |
| Volume | 34 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - Jan 1 2018 |
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All Science Journal Classification (ASJC) codes
- Analytical Chemistry
Cite this
A dual alkylated peptide-ligand enhances affinity to human serum albumin. / Nakhaei, Elnaz; Takehara, Ko; Sato, Hikari; Zai, Khadijah; Kishimura, Akihiro; Mori, Takeshi; Katayama, Yoshiki.
In: analytical sciences, Vol. 34, No. 4, 01.01.2018, p. 501-504.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A dual alkylated peptide-ligand enhances affinity to human serum albumin
AU - Nakhaei, Elnaz
AU - Takehara, Ko
AU - Sato, Hikari
AU - Zai, Khadijah
AU - Kishimura, Akihiro
AU - Mori, Takeshi
AU - Katayama, Yoshiki
PY - 2018/1/1
Y1 - 2018/1/1
N2 - Therapeutic peptides and diagnostic agents with their molecular size below the renal clearance threshold suffer from short blood circulation time. Here, we report a novel design of peptide-based ligand with a strong binding affinity to human serum albumin (HSA), which can be used as a tag to extend the blood circulation of small-size molecules. We designed ligands with dual alkyl groups connected with a negatively charged spacer. The ligands showed both higher binding affinity to HSA and a higher retention in mice blood than that of a single alkylated peptide.
AB - Therapeutic peptides and diagnostic agents with their molecular size below the renal clearance threshold suffer from short blood circulation time. Here, we report a novel design of peptide-based ligand with a strong binding affinity to human serum albumin (HSA), which can be used as a tag to extend the blood circulation of small-size molecules. We designed ligands with dual alkyl groups connected with a negatively charged spacer. The ligands showed both higher binding affinity to HSA and a higher retention in mice blood than that of a single alkylated peptide.
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U2 - 10.2116/analsci.17P614
DO - 10.2116/analsci.17P614
M3 - Article
VL - 34
SP - 501
EP - 504
JO - Analytical Sciences
JF - Analytical Sciences
SN - 0910-6340
IS - 4
ER -