A FancD2-monoubiquitin fusion reveals hidden functions of Fanconi anemia core complex in DNA repair

Nobuko Matsushita, Hiroyuki Kitao, Masamichi Ishiai, Naoki Nagashima, Seiki Hirano, Katsuya Okawa, Tomohiko Ohta, David S. Yu, Peter J. McHugh, Ian D. Hickson, Ashok R. Venkitaraman, Hitoshi Kurumizaka, Minoru Takata

Research output: Contribution to journalArticle

108 Citations (Scopus)

Abstract

In DNA damage responses, the Fanconi anemia (FA) protein, FancD2, is targeted to chromatin and forms nuclear foci following its monoubiquitination, a process likely catalyzed by the FA core complex. Here, we show that a chicken FancD2-ubiquitin fusion protein, carrying a Lys-Arg substitution removing the natural monoubiquitination site (D2KR-Ub), could reverse cisplatin hypersensitivity and localize to chromatin in FANCD2-deficient DT40 cells. Importantly, the chromatin targeting was dependent on three core complex components as well as the hydrophobic surface of ubiquitin that may direct protein-protein interactions. Furthermore, a constitutively chromatin bound fusion of D2KR-histone H2B could complement cisplatin sensitivity in FANCD2- but not FANCC-, FANCG-, or FANCL-deficient cells. Thus these core complex components have an additional function in the DNA repair, which is independent of the monoubiquitination and chromatin targeting of FancD2. These results define functional consequences of FancD2 monoubiquitination and reveal previously hidden functions for the FA protein core complex.

Original languageEnglish
Pages (from-to)841-847
Number of pages7
JournalMolecular Cell
Volume19
Issue number6
DOIs
Publication statusPublished - Sep 16 2005
Externally publishedYes

Fingerprint

Fanconi Anemia
DNA Repair
Chromatin
Fanconi Anemia Complementation Group Proteins
Ubiquitin
Cisplatin
Proteins
Histones
DNA Damage
Chickens
Hypersensitivity

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

Cite this

A FancD2-monoubiquitin fusion reveals hidden functions of Fanconi anemia core complex in DNA repair. / Matsushita, Nobuko; Kitao, Hiroyuki; Ishiai, Masamichi; Nagashima, Naoki; Hirano, Seiki; Okawa, Katsuya; Ohta, Tomohiko; Yu, David S.; McHugh, Peter J.; Hickson, Ian D.; Venkitaraman, Ashok R.; Kurumizaka, Hitoshi; Takata, Minoru.

In: Molecular Cell, Vol. 19, No. 6, 16.09.2005, p. 841-847.

Research output: Contribution to journalArticle

Matsushita, N, Kitao, H, Ishiai, M, Nagashima, N, Hirano, S, Okawa, K, Ohta, T, Yu, DS, McHugh, PJ, Hickson, ID, Venkitaraman, AR, Kurumizaka, H & Takata, M 2005, 'A FancD2-monoubiquitin fusion reveals hidden functions of Fanconi anemia core complex in DNA repair', Molecular Cell, vol. 19, no. 6, pp. 841-847. https://doi.org/10.1016/j.molcel.2005.08.018
Matsushita, Nobuko ; Kitao, Hiroyuki ; Ishiai, Masamichi ; Nagashima, Naoki ; Hirano, Seiki ; Okawa, Katsuya ; Ohta, Tomohiko ; Yu, David S. ; McHugh, Peter J. ; Hickson, Ian D. ; Venkitaraman, Ashok R. ; Kurumizaka, Hitoshi ; Takata, Minoru. / A FancD2-monoubiquitin fusion reveals hidden functions of Fanconi anemia core complex in DNA repair. In: Molecular Cell. 2005 ; Vol. 19, No. 6. pp. 841-847.
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