A fatal case of poisoning related to new cathinone designer drugs, 4-methoxy PV8, PV9, and 4-methoxy PV9, and a dissociative agent, diphenidine

Keiko Kudo, Yosuke Usumoto, Ruri Kikura-Hanajiri, Naomi Sameshima, Akiko Tsuji, Noriaki Ikeda

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

A woman in her thirties was found dead on a bed. Considerable amounts of "aroma liquid" and "bath salt" products and hypnotic drug tablets were scattered beside the bed. Autopsy showed pulmonary congestion and edema. Gas chromatography-mass spectrometry (GC-MS) and liquid chromatography-mass spectrometry (LC-MS) analyses of "aroma liquid" and "bath salt" products showed the presence of new cathinone designer drugs, 4-methoxy PV8 (4-methoxy PHPP), PV9 (α-POP), and 4-methoxy PV9 (4-methoxy α-POP), and a dissociative agent, diphenidine. Drug screening in stomach contents, blood and hydrolyzed urine of the woman by GC-MS and liquid chromatography-tandem mass spectrometry (LC-MS/MS) revealed the presence of the above 4 types of drugs and 3 types of benzodiazepines, triazolam, flunitrazepam, and nitrazepam, and their metabolites. The above 7 drugs and 3 benzodiazepine metabolites were simultaneously determined by LC-MS/MS after modified QuEChERS (Quick, Easy, Cheap, Effective, Rugged, Safe) extraction using diazepam-d5 as the internal standard. The concentrations of 4-methoxy PV8, PV9, 4-methoxy PV9, and diphenidine in the femoral blood were 2.69, 0.743, 0.261, and 1.38μg/ml, respectively, which were significantly higher than concentrations reported in previous cases. Alcohol concentration in the femoral blood was 1.52mg/ml. Based on the pathological and toxicological findings, the cause of death was determined to be 3 types of cathinone drugs, 4-methoxy PV8, PV9, and 4-methoxy PV9, and diphenidine poisoning under the influence of 3 benzodiazepines and alcohol.

Original languageEnglish
Pages (from-to)421-426
Number of pages6
JournalLegal Medicine
Volume17
Issue number5
DOIs
Publication statusPublished - Sep 1 2015

Fingerprint

Designer Drugs
Liquid Chromatography
Poisoning
Benzodiazepines
Mass Spectrometry
Thigh
Baths
Pharmaceutical Preparations
Gas Chromatography-Mass Spectrometry
Salts
Alcohols
Nitrazepam
Triazolam
Flunitrazepam
Preclinical Drug Evaluations
Gastrointestinal Contents
Pulmonary Edema
Diazepam
Tandem Mass Spectrometry
Hypnotics and Sedatives

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine
  • Issues, ethics and legal aspects

Cite this

A fatal case of poisoning related to new cathinone designer drugs, 4-methoxy PV8, PV9, and 4-methoxy PV9, and a dissociative agent, diphenidine. / Kudo, Keiko; Usumoto, Yosuke; Kikura-Hanajiri, Ruri; Sameshima, Naomi; Tsuji, Akiko; Ikeda, Noriaki.

In: Legal Medicine, Vol. 17, No. 5, 01.09.2015, p. 421-426.

Research output: Contribution to journalArticle

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