Abstract
A fluorescent-labeled oligopeptide (DACM-CLRRASLK-fluorescein), containing a consensus amino acid sequence (RRXSL) of cyclic AMP (cAMP) dependent protein kinase A (PKA) substrate-proteins, was designed. The fluorescent peptide was a good substrate of PKA, and the phosphorylation of its serin residue caused an intensive change in fluorescent intensity. We expect that the peptide will be useful as a fluorescent indicator for monitoring PKA activity in living cells.
| Original language | English |
|---|---|
| Pages (from-to) | 1905-1907 |
| Number of pages | 3 |
| Journal | Analyst |
| Volume | 125 |
| Issue number | 11 |
| DOIs | |
| Publication status | Published - Jan 1 2000 |
All Science Journal Classification (ASJC) codes
- Analytical Chemistry
- Biochemistry
- Environmental Chemistry
- Spectroscopy
- Electrochemistry
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Cite this
Ohuchi, Y., Katayama, Y., & Maeda, M. (2000). A fluorescent-labeled oligopeptide for monitoring PKA-mediated phosphorylation. Analyst, 125(11), 1905-1907. https://doi.org/10.1039/b007152h