Abstract
A fluorescent-labeled oligopeptide (DACM-CLRRASLK-fluorescein), containing a consensus amino acid sequence (RRXSL) of cyclic AMP (cAMP) dependent protein kinase A (PKA) substrate-proteins, was designed. The fluorescent peptide was a good substrate of PKA, and the phosphorylation of its serin residue caused an intensive change in fluorescent intensity. We expect that the peptide will be useful as a fluorescent indicator for monitoring PKA activity in living cells.
| Original language | English |
|---|---|
| Pages (from-to) | 1905-1907 |
| Number of pages | 3 |
| Journal | Analyst |
| Volume | 125 |
| Issue number | 11 |
| DOIs | |
| Publication status | Published - Dec 4 2000 |
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All Science Journal Classification (ASJC) codes
- Analytical Chemistry
- Biochemistry
- Environmental Chemistry
- Spectroscopy
- Electrochemistry
Cite this
A fluorescent-labeled oligopeptide for monitoring PKA-mediated phosphorylation. / Ohuchi, Y.; Katayama, Yoshiki; Maeda, M.
In: Analyst, Vol. 125, No. 11, 04.12.2000, p. 1905-1907.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A fluorescent-labeled oligopeptide for monitoring PKA-mediated phosphorylation
AU - Ohuchi, Y.
AU - Katayama, Yoshiki
AU - Maeda, M.
PY - 2000/12/4
Y1 - 2000/12/4
N2 - A fluorescent-labeled oligopeptide (DACM-CLRRASLK-fluorescein), containing a consensus amino acid sequence (RRXSL) of cyclic AMP (cAMP) dependent protein kinase A (PKA) substrate-proteins, was designed. The fluorescent peptide was a good substrate of PKA, and the phosphorylation of its serin residue caused an intensive change in fluorescent intensity. We expect that the peptide will be useful as a fluorescent indicator for monitoring PKA activity in living cells.
AB - A fluorescent-labeled oligopeptide (DACM-CLRRASLK-fluorescein), containing a consensus amino acid sequence (RRXSL) of cyclic AMP (cAMP) dependent protein kinase A (PKA) substrate-proteins, was designed. The fluorescent peptide was a good substrate of PKA, and the phosphorylation of its serin residue caused an intensive change in fluorescent intensity. We expect that the peptide will be useful as a fluorescent indicator for monitoring PKA activity in living cells.
UR - http://www.scopus.com/inward/record.url?scp=0033709914&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0033709914&partnerID=8YFLogxK
U2 - 10.1039/b007152h
DO - 10.1039/b007152h
M3 - Article
C2 - 11193075
AN - SCOPUS:0033709914
VL - 125
SP - 1905
EP - 1907
JO - The Analyst
JF - The Analyst
SN - 0003-2654
IS - 11
ER -