A fluorescent-labeled oligopeptide for monitoring PKA-mediated phosphorylation

Y. Ohuchi, Y. Katayama, M. Maeda

    Research output: Contribution to journalArticlepeer-review

    6 Citations (Scopus)

    Abstract

    A fluorescent-labeled oligopeptide (DACM-CLRRASLK-fluorescein), containing a consensus amino acid sequence (RRXSL) of cyclic AMP (cAMP) dependent protein kinase A (PKA) substrate-proteins, was designed. The fluorescent peptide was a good substrate of PKA, and the phosphorylation of its serin residue caused an intensive change in fluorescent intensity. We expect that the peptide will be useful as a fluorescent indicator for monitoring PKA activity in living cells.

    Original languageEnglish
    Pages (from-to)1905-1907
    Number of pages3
    JournalAnalyst
    Volume125
    Issue number11
    DOIs
    Publication statusPublished - Jan 1 2000

    All Science Journal Classification (ASJC) codes

    • Analytical Chemistry
    • Biochemistry
    • Environmental Chemistry
    • Spectroscopy
    • Electrochemistry

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