A fluorescent-labeled oligopeptide for monitoring PKA-mediated phosphorylation

Y. Ohuchi; Y. Katayama; M. Maeda

doi:10.1039/b007152h

A fluorescent-labeled oligopeptide for monitoring PKA-mediated phosphorylation

Y. Ohuchi, Y. Katayama, M. Maeda

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6 Citations (Scopus)

Abstract

A fluorescent-labeled oligopeptide (DACM-CLRRASLK-fluorescein), containing a consensus amino acid sequence (RRXSL) of cyclic AMP (cAMP) dependent protein kinase A (PKA) substrate-proteins, was designed. The fluorescent peptide was a good substrate of PKA, and the phosphorylation of its serin residue caused an intensive change in fluorescent intensity. We expect that the peptide will be useful as a fluorescent indicator for monitoring PKA activity in living cells.

Original language	English
Pages (from-to)	1905-1907
Number of pages	3
Journal	Analyst
Volume	125
Issue number	11
DOIs	https://doi.org/10.1039/b007152h
Publication status	Published - Jan 1 2000

All Science Journal Classification (ASJC) codes

Analytical Chemistry
Biochemistry
Environmental Chemistry
Spectroscopy
Electrochemistry

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abstract = "A fluorescent-labeled oligopeptide (DACM-CLRRASLK-fluorescein), containing a consensus amino acid sequence (RRXSL) of cyclic AMP (cAMP) dependent protein kinase A (PKA) substrate-proteins, was designed. The fluorescent peptide was a good substrate of PKA, and the phosphorylation of its serin residue caused an intensive change in fluorescent intensity. We expect that the peptide will be useful as a fluorescent indicator for monitoring PKA activity in living cells.",

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AU - Maeda, M.

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AB - A fluorescent-labeled oligopeptide (DACM-CLRRASLK-fluorescein), containing a consensus amino acid sequence (RRXSL) of cyclic AMP (cAMP) dependent protein kinase A (PKA) substrate-proteins, was designed. The fluorescent peptide was a good substrate of PKA, and the phosphorylation of its serin residue caused an intensive change in fluorescent intensity. We expect that the peptide will be useful as a fluorescent indicator for monitoring PKA activity in living cells.

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A fluorescent-labeled oligopeptide for monitoring PKA-mediated phosphorylation

Abstract

All Science Journal Classification (ASJC) codes

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