A fluorescent-labeled oligopeptide for monitoring PKA-mediated phosphorylation

Y. Ohuchi, Y. Katayama, M. Maeda

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

A fluorescent-labeled oligopeptide (DACM-CLRRASLK-fluorescein), containing a consensus amino acid sequence (RRXSL) of cyclic AMP (cAMP) dependent protein kinase A (PKA) substrate-proteins, was designed. The fluorescent peptide was a good substrate of PKA, and the phosphorylation of its serin residue caused an intensive change in fluorescent intensity. We expect that the peptide will be useful as a fluorescent indicator for monitoring PKA activity in living cells.

Original languageEnglish
Pages (from-to)1905-1907
Number of pages3
JournalAnalyst
Volume125
Issue number11
DOIs
Publication statusPublished - Jan 1 2000

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy
  • Electrochemistry

Fingerprint Dive into the research topics of 'A fluorescent-labeled oligopeptide for monitoring PKA-mediated phosphorylation'. Together they form a unique fingerprint.

  • Cite this