A focused microarray for screening rat embryonic stem cell lines

James Hong, Hong He, Phuoc Bui, Ben Ryba-White, Mohammad A.K. Rumi, Michael J. Soares, Debasree Dutta, Soumen Paul, Masaki Kawamata, Takahiro Ochiya, Qi Long Ying, Pavan Rajanahalli, Mark L. Weiss

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Abstract

Here, we describe a focused microarray for screening rat embryonic stem cells (ESCs) and provide validation data that this array can distinguish undifferentiated rat ESCs from rat trophoblast stem (TS) cells, rat extraembryonic endoderm cells, mouse embryonic fibroblast feeder cells, and differentiated rat ESCs. Using this tool, genuine rat ESC lines, which have been expanded in a conventional rat ESC medium containing two inhibitors (2i), for example, glycogen synthase kinase 3 (GSK3) and mitogen-activated protein kinase (MEK) inhibitors, and leukemia inhibitory factor, and genuine rat ESCs, which have been expanded in rat ESC medium containing four inhibitors (4i), for example, GSK3, MEK, Alk5, and Rho-associated kinase inhibitors were compared; as were genuine rat ESCs from 4 different strains of rats. Expression of Cdx2, a gene associated with trophoblast determination, was observed in genuine, undifferentiated rat ESCs from 4 strains and from both 2i and 4i ESC derivation medium. This finding is in contrast to undifferentiated mouse ESCs that do not express Cdx2. The rat ESC focused microarray described in this report has utility for rapid screening of rat ESCs. This tool will enable optimization of culture conditions in the future.

Original languageEnglish
Pages (from-to)431-443
Number of pages13
JournalStem cells and development
Volume22
Issue number3
DOIs
Publication statusPublished - Feb 1 2013
Externally publishedYes

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All Science Journal Classification (ASJC) codes

  • Hematology
  • Developmental Biology
  • Cell Biology

Cite this

Hong, J., He, H., Bui, P., Ryba-White, B., Rumi, M. A. K., Soares, M. J., ... Weiss, M. L. (2013). A focused microarray for screening rat embryonic stem cell lines. Stem cells and development, 22(3), 431-443. https://doi.org/10.1089/scd.2012.0279