A fourier transform infrared spectroscopic study of the molecular interaction in synthetic bilayer membrane

I. Shimada, H. Ishida, A. Ishitani, T. Kunitake

Research output: Contribution to journalArticle

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Abstract

Fourier transform infrared spectroscopy is used to study the molecular interaction between gramicidin D and bilayer membranes, dioctadecadimethylammonium bromide (2C18N+2C1Br-), and 1,2-di-palmytoyl-l-α-phosphatidylcholine (DPPC). Frequencies and bandwidths of the symmetric CH2 stretching band measured as a function of temperature are used to study variation of packing of alkyl chains. The bilayer membrane prepared from 2C18N+2C1Br- is found to have a gel to liquid crystal phase transition at 42°C. The presence of gramicidin in the membrane causes an increase in the mobility of the alkyl chain and also a decrease in the abruptness of the transition. The frequencies of amide I and II bands of gramicidin reflecting secondary structures of polypeptides are used to identify its conformation in membranes and to study the interaction between gramicidin and the matrices. Gramicidin is found to have hydrophobic interaction with 2C18N+2C1Br-, whereas it has both hydrophobic and hydrophilic interactions with DPPC.

Original languageEnglish
Pages (from-to)523-528
Number of pages6
JournalJournal of Colloid And Interface Science
Volume120
Issue number2
DOIs
Publication statusPublished - Dec 1987

Fingerprint

Gramicidin
Molecular interactions
Fourier transforms
Infrared radiation
Membranes
Phosphatidylcholines
Polypeptides
Liquid Crystals
Amides
Liquid crystals
Stretching
Fourier transform infrared spectroscopy
Conformations
Bromides
Gels
Phase transitions
Bandwidth
Peptides
Temperature

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Surfaces, Coatings and Films
  • Colloid and Surface Chemistry

Cite this

A fourier transform infrared spectroscopic study of the molecular interaction in synthetic bilayer membrane. / Shimada, I.; Ishida, H.; Ishitani, A.; Kunitake, T.

In: Journal of Colloid And Interface Science, Vol. 120, No. 2, 12.1987, p. 523-528.

Research output: Contribution to journalArticle

Shimada, I. ; Ishida, H. ; Ishitani, A. ; Kunitake, T. / A fourier transform infrared spectroscopic study of the molecular interaction in synthetic bilayer membrane. In: Journal of Colloid And Interface Science. 1987 ; Vol. 120, No. 2. pp. 523-528.
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