A frameshift mutation of the chloroplast mat K coding region is associated with chlorophyll deficiency in the Cryptomeria japonica virescent mutant Wogon-Sugi

Tomonori Hirao, Atsushi Watanabe, Manabu Kurita, Teiji Kondo, Katsuhiko Takata

Research output: Contribution to journalReview article

11 Citations (Scopus)

Abstract

Wogon-Sugi has been reported as a cytoplasmically inherited virescent mutant selected from a horticultural variety of Cryptomeria japonica. Although previous studies of plastid structure and inheritance indicated that at least some mutations are encoded by the chloroplast genome, the causative gene responsible for the primary chlorophyll deficiency in Wogon-Sugi, has not been identified. In this study, we identified this gene by genomic sequencing of chloroplast DNA and genetic analysis. Chloroplast DNA sequencing of 16 wild-type and 16 Wogon-Sugi plants showed a 19-bp insertional sequence in the mat K coding region in the Wogon-Sugi. This insertion disrupted the mat K reading frame. Although an indel mutation in the ycf 1 and ycf 2 coding region was detected in Wogon-Sugi, sequence variations similar to that of wogon-Sugi were also detected in several wild-type lines, and they maintained the reading frame. Genetic analysis of the 19 bp insertional mutation in the mat K coding region showed that it was found only in the chlorophyll-deficient sector of 125 full-sibling seedlings. Therefore, the 19-bp insertion in the mat K coding region is the most likely candidate at present for a mutation underlying the Wogon-Sugi phenotype.

Original languageEnglish
Pages (from-to)311-321
Number of pages11
JournalCurrent Genetics
Volume55
Issue number3
DOIs
Publication statusPublished - May 18 2009

Fingerprint

Cryptomeria
Frameshift Mutation
Chloroplasts
Chlorophyll
Chloroplast DNA
Reading Frames
Mutation
Chloroplast Genome
Plastids
DNA Sequence Analysis
Seedlings
Genes
Phenotype

All Science Journal Classification (ASJC) codes

  • Genetics

Cite this

A frameshift mutation of the chloroplast mat K coding region is associated with chlorophyll deficiency in the Cryptomeria japonica virescent mutant Wogon-Sugi. / Hirao, Tomonori; Watanabe, Atsushi; Kurita, Manabu; Kondo, Teiji; Takata, Katsuhiko.

In: Current Genetics, Vol. 55, No. 3, 18.05.2009, p. 311-321.

Research output: Contribution to journalReview article

@article{e5429613a9634a7eabc1d71ed96333c3,
title = "A frameshift mutation of the chloroplast mat K coding region is associated with chlorophyll deficiency in the Cryptomeria japonica virescent mutant Wogon-Sugi",
abstract = "Wogon-Sugi has been reported as a cytoplasmically inherited virescent mutant selected from a horticultural variety of Cryptomeria japonica. Although previous studies of plastid structure and inheritance indicated that at least some mutations are encoded by the chloroplast genome, the causative gene responsible for the primary chlorophyll deficiency in Wogon-Sugi, has not been identified. In this study, we identified this gene by genomic sequencing of chloroplast DNA and genetic analysis. Chloroplast DNA sequencing of 16 wild-type and 16 Wogon-Sugi plants showed a 19-bp insertional sequence in the mat K coding region in the Wogon-Sugi. This insertion disrupted the mat K reading frame. Although an indel mutation in the ycf 1 and ycf 2 coding region was detected in Wogon-Sugi, sequence variations similar to that of wogon-Sugi were also detected in several wild-type lines, and they maintained the reading frame. Genetic analysis of the 19 bp insertional mutation in the mat K coding region showed that it was found only in the chlorophyll-deficient sector of 125 full-sibling seedlings. Therefore, the 19-bp insertion in the mat K coding region is the most likely candidate at present for a mutation underlying the Wogon-Sugi phenotype.",
author = "Tomonori Hirao and Atsushi Watanabe and Manabu Kurita and Teiji Kondo and Katsuhiko Takata",
year = "2009",
month = "5",
day = "18",
doi = "10.1007/s00294-009-0247-9",
language = "English",
volume = "55",
pages = "311--321",
journal = "Current Genetics",
issn = "0172-8083",
publisher = "Springer Verlag",
number = "3",

}

TY - JOUR

T1 - A frameshift mutation of the chloroplast mat K coding region is associated with chlorophyll deficiency in the Cryptomeria japonica virescent mutant Wogon-Sugi

AU - Hirao, Tomonori

AU - Watanabe, Atsushi

AU - Kurita, Manabu

AU - Kondo, Teiji

AU - Takata, Katsuhiko

PY - 2009/5/18

Y1 - 2009/5/18

N2 - Wogon-Sugi has been reported as a cytoplasmically inherited virescent mutant selected from a horticultural variety of Cryptomeria japonica. Although previous studies of plastid structure and inheritance indicated that at least some mutations are encoded by the chloroplast genome, the causative gene responsible for the primary chlorophyll deficiency in Wogon-Sugi, has not been identified. In this study, we identified this gene by genomic sequencing of chloroplast DNA and genetic analysis. Chloroplast DNA sequencing of 16 wild-type and 16 Wogon-Sugi plants showed a 19-bp insertional sequence in the mat K coding region in the Wogon-Sugi. This insertion disrupted the mat K reading frame. Although an indel mutation in the ycf 1 and ycf 2 coding region was detected in Wogon-Sugi, sequence variations similar to that of wogon-Sugi were also detected in several wild-type lines, and they maintained the reading frame. Genetic analysis of the 19 bp insertional mutation in the mat K coding region showed that it was found only in the chlorophyll-deficient sector of 125 full-sibling seedlings. Therefore, the 19-bp insertion in the mat K coding region is the most likely candidate at present for a mutation underlying the Wogon-Sugi phenotype.

AB - Wogon-Sugi has been reported as a cytoplasmically inherited virescent mutant selected from a horticultural variety of Cryptomeria japonica. Although previous studies of plastid structure and inheritance indicated that at least some mutations are encoded by the chloroplast genome, the causative gene responsible for the primary chlorophyll deficiency in Wogon-Sugi, has not been identified. In this study, we identified this gene by genomic sequencing of chloroplast DNA and genetic analysis. Chloroplast DNA sequencing of 16 wild-type and 16 Wogon-Sugi plants showed a 19-bp insertional sequence in the mat K coding region in the Wogon-Sugi. This insertion disrupted the mat K reading frame. Although an indel mutation in the ycf 1 and ycf 2 coding region was detected in Wogon-Sugi, sequence variations similar to that of wogon-Sugi were also detected in several wild-type lines, and they maintained the reading frame. Genetic analysis of the 19 bp insertional mutation in the mat K coding region showed that it was found only in the chlorophyll-deficient sector of 125 full-sibling seedlings. Therefore, the 19-bp insertion in the mat K coding region is the most likely candidate at present for a mutation underlying the Wogon-Sugi phenotype.

UR - http://www.scopus.com/inward/record.url?scp=67349184029&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=67349184029&partnerID=8YFLogxK

U2 - 10.1007/s00294-009-0247-9

DO - 10.1007/s00294-009-0247-9

M3 - Review article

C2 - 19449186

AN - SCOPUS:67349184029

VL - 55

SP - 311

EP - 321

JO - Current Genetics

JF - Current Genetics

SN - 0172-8083

IS - 3

ER -