A G-protein of sarcoplasmic reticulum of skeletal muscle is activated by caffeine or inositol trisphosphate

Tohru Hasegawa, Shuzo Kumagai

Research output: Contribution to journal › Article

4 Citations (Scopus)

Abstract

Here we show the activation of G-protein by inositol trisphosphate (IP3) or caffeine in sarcoplasmic reticulum (SR) of skeletal muscle and the consequent existence of a common mechanism of Ca2+ release from SR induced by caffeine and by IP3. (i) Indomethacin inhibits Ca2+ release induced by IP3 or caffeine. (ii) PGE1 does not induce Ca2+ release itself, but does stimulate Ca2+ release induced by IP3, or caffeine, from SR. (iii) Forskolin stimulates both types of Ca2+ release. The inhibitory effect of indomethacin on both forms of Ca2+ release, and the stimulatory effect of PGE1 and forskolin on either Ca2+ release suggest that there exists a common mechanism between IP3- and caffeine-induced Ca2+ release. (iv) Caffeine or IP3 activates G-protein via inhibition of a GTPase activity. (v) Indomethacin itself inactivates this G-protein by stimulation of a GTPase activity and reverses the activation of G-protein induced by IP3 or caffeine. (vi) PGE1 competes with the inhibitory effect of indomethacin on GTPase activity and PGE1 itself activates G-protein through inhibition of GTPase activity. From these results, it could be suggested that caffeine or IP3 induces Ca2+ release from the SR via activation of G-protein, which affects the Ca2+ channel and cAMP which seems to affect G-protein via A-kinase.

Original languageEnglish
Pages (from-to)283-286
Number of pages4
JournalFEBS Letters
Volume244
Issue number2
DOIs
Publication statusPublished - Feb 27 1989
Externally publishedYes

Fingerprint

Sarcoplasmic Reticulum
Inositol
Caffeine
GTP-Binding Proteins
Muscle
Skeletal Muscle
Alprostadil
GTP Phosphohydrolases
Indomethacin
Chemical activation
Colforsin
Phosphotransferases

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

A G-protein of sarcoplasmic reticulum of skeletal muscle is activated by caffeine or inositol trisphosphate. / Hasegawa, Tohru; Kumagai, Shuzo.

In: FEBS Letters, Vol. 244, No. 2, 27.02.1989, p. 283-286.

Research output: Contribution to journal › Article

Hasegawa, T & Kumagai, S 1989, 'A G-protein of sarcoplasmic reticulum of skeletal muscle is activated by caffeine or inositol trisphosphate', FEBS Letters, vol. 244, no. 2, pp. 283-286. https://doi.org/10.1016/0014-5793(89)80546-0
Hasegawa T, Kumagai S. A G-protein of sarcoplasmic reticulum of skeletal muscle is activated by caffeine or inositol trisphosphate. FEBS Letters. 1989 Feb 27;244(2):283-286. https://doi.org/10.1016/0014-5793(89)80546-0
Hasegawa, Tohru ; Kumagai, Shuzo. / A G-protein of sarcoplasmic reticulum of skeletal muscle is activated by caffeine or inositol trisphosphate. In: FEBS Letters. 1989 ; Vol. 244, No. 2. pp. 283-286.
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