A gene cluster for the synthesis of serotype d-specific polysaccharide antigen in Actinobacillus actinomycetemcomitans

Yoshio Nakano, Yasuo Yoshida, Nao Suzuki, Yoshihisa Yamashita, Toshihiko Koga

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

The serotype d antigen of Actinobacillus actinomycetemcomitans consists of d-glucose, d-mannose, and l-rhamnose in a molar ratio of 1:2:1. A gene cluster involved in the synthesis of serotype-specific polysaccharide antigen was cloned from the chromosomal DNA of A. actinomycetemcomitans IDH 781 (serotype d). This cluster consisted of 12 open reading frames. Insertional inactivation of six genes in this cluster resulted in loss of ability of A. actinomycetemcomitans IDH 781 cells to produce the polysaccharide. Comparing the structure of the gene cluster with similar clusters from other serotypes of A. actinomycetemcomitans, showed that eight genes are unique to serotype d; the other four genes are involved in the biosynthesis of dTDP-l-rhamnose. These results suggest that the synthesis and structure of serotype d-specific polysaccharide of A. actinomycetemcomitans is quite different from those of other serotype strains. (C) 2000 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)259-263
Number of pages5
JournalBiochimica et Biophysica Acta - Gene Structure and Expression
Volume1493
Issue number1-2
DOIs
Publication statusPublished - Sep 7 2000

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Aggregatibacter actinomycetemcomitans
Multigene Family
Polysaccharides
Genes
Antigens
Rhamnose
Biosynthesis
Mannose
Serogroup
Open Reading Frames
Glucose
DNA

All Science Journal Classification (ASJC) codes

  • Structural Biology
  • Biophysics
  • Biochemistry
  • Genetics

Cite this

A gene cluster for the synthesis of serotype d-specific polysaccharide antigen in Actinobacillus actinomycetemcomitans. / Nakano, Yoshio; Yoshida, Yasuo; Suzuki, Nao; Yamashita, Yoshihisa; Koga, Toshihiko.

In: Biochimica et Biophysica Acta - Gene Structure and Expression, Vol. 1493, No. 1-2, 07.09.2000, p. 259-263.

Research output: Contribution to journalArticle

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