A gram-negative characteristic segment in Escherichia coli DnaK is essential for the ATP-dependent cooperative function with the co-chaperones DnaJ and GrpE

Shinya Sugimoto, Chihana Higashi, Kozue Saruwatari, Jiro Nakayama, Kenji Sonomoto

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

We describe importance of the characteristic segment in ATPase domain of DnaK chaperone which is present in all gram-negative bacteria but is absent in all gram-positive bacteria. In vitro studies, ATPase activity, luciferase-refolding activity, and surface plasmon resonance analyses, demonstrated that a segment-deletion mutant DnaKΔ74-96 became defective in the cooperation with the co-chaperones DnaJ and GrpE. In addition, in vivo complementation assay showed that expression of DnaKΔ74-96 could not rescue the viability of Escherichia coli ΔdnaK mutant at 43 °C. Consequently, we suggest evolutionary significance for this DnaK ATPase domain segment in gram-negative bacteria towards the DnaK chaperone system.

Original languageEnglish
Pages (from-to)2993-2999
Number of pages7
JournalFEBS Letters
Volume581
Issue number16
DOIs
Publication statusPublished - Jun 26 2007

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Escherichia coli
Adenosine Triphosphatases
Bacteria
Adenosine Triphosphate
Gram-Negative Bacteria
Surface Plasmon Resonance
Gram-Positive Bacteria
Surface plasmon resonance
Luciferases
Assays
In Vitro Techniques

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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A gram-negative characteristic segment in Escherichia coli DnaK is essential for the ATP-dependent cooperative function with the co-chaperones DnaJ and GrpE. / Sugimoto, Shinya; Higashi, Chihana; Saruwatari, Kozue; Nakayama, Jiro; Sonomoto, Kenji.

In: FEBS Letters, Vol. 581, No. 16, 26.06.2007, p. 2993-2999.

Research output: Contribution to journalArticle

Sugimoto, Shinya ; Higashi, Chihana ; Saruwatari, Kozue ; Nakayama, Jiro ; Sonomoto, Kenji. / A gram-negative characteristic segment in Escherichia coli DnaK is essential for the ATP-dependent cooperative function with the co-chaperones DnaJ and GrpE. In: FEBS Letters. 2007 ; Vol. 581, No. 16. pp. 2993-2999.
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