TY - JOUR
T1 - A green and effective method using oils to remove chlorophyll from chromolaena odorata (L.) R.M. King & H. Rob
AU - Phaisan, Suppalak
AU - Yusakul, Gorawit
AU - Sakdamas, Attapon
AU - Taluengjit, Nattapon
AU - Sakamoto, Seiichi
AU - Putalun, Waraporn
N1 - Funding Information:
We would like to acknowledge the support from The Thailand Research Fund [grant number IRN 61W0005] and School of Pharmacy, Walailak University, Thailand. This research was also partially supported by the Research Fund [grant number WU61313] from the Institute of Research and Innovation, Walailak University. The authors also would like to thank the facilities and support of The Center for Scientific and Technological Equipment, Walailak University, Thailand.
Publisher Copyright:
© 2020, Prince of Songkla University. All rights reserved.
PY - 2020
Y1 - 2020
N2 - Chromolaena odorata (L.) R.M. King & H. Rob has excellent wound healing effects. Therefore, it is useful for cosmeceutical purposes; however, the dark-greenish appearance of its extract limits its attractiveness for product development. This study aimed to develop an oil-based system for chlorophyll removal. Dried ethanolic extracts were dissolved in different aqueous EtOH solvents, and then the chlorophyll was partitioned to the oil phase. The efficiency of chlorophyll removal was monitored using a spectrophotometer. The recoveries of flavonoid and phenolic contents and the antioxidant activity were determined after the treatment. When the solutions of extract were prepared in 25% (v/v)-75% (v/v) EtOH in water, the efficacy of chlorophyll removal by oil was higher than 85%. A higher concentration of EtOH resulted in a lower chlorophyll removal efficiency; however, the recoveries of phenolic and flavonoid contents and antioxidant activity improved. Palm oil showed efficiency higher than hexane for chlorophyll removal and high recoveries of the beneficial phytochemicals. This system can be applied in the fields of natural products-based health product development and in phytochemical studies.
AB - Chromolaena odorata (L.) R.M. King & H. Rob has excellent wound healing effects. Therefore, it is useful for cosmeceutical purposes; however, the dark-greenish appearance of its extract limits its attractiveness for product development. This study aimed to develop an oil-based system for chlorophyll removal. Dried ethanolic extracts were dissolved in different aqueous EtOH solvents, and then the chlorophyll was partitioned to the oil phase. The efficiency of chlorophyll removal was monitored using a spectrophotometer. The recoveries of flavonoid and phenolic contents and the antioxidant activity were determined after the treatment. When the solutions of extract were prepared in 25% (v/v)-75% (v/v) EtOH in water, the efficacy of chlorophyll removal by oil was higher than 85%. A higher concentration of EtOH resulted in a lower chlorophyll removal efficiency; however, the recoveries of phenolic and flavonoid contents and antioxidant activity improved. Palm oil showed efficiency higher than hexane for chlorophyll removal and high recoveries of the beneficial phytochemicals. This system can be applied in the fields of natural products-based health product development and in phytochemical studies.
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M3 - Article
AN - SCOPUS:85086864691
SN - 0125-3395
VL - 42
SP - 1084
EP - 1090
JO - Songklanakarin Journal of Science and Technology
JF - Songklanakarin Journal of Science and Technology
IS - 5
ER -