A harmonized immunoassay with liquid chromatography-mass spectrometry analysis in egg allergen determination

Masaomi Nimata, Hideki Okada, Kei Kurihara, Tsukasa Sugimoto, Tsutomu Honjoh, Kazuhiko Kuroda, Takeo Yano, Hirofumi Tachibana, Masahiro Shoji

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Food allergy is a serious health issue worldwide. Implementing allergen labeling regulations is extremely challenging for regulators, food manufacturers, and analytical kit manufacturers. Here we have developed an “amino acid sequence immunoassay” approach to ELISA. The new ELISA comprises of a monoclonal antibody generated via an analyte specific peptide antigen and sodium lauryl sulfate/sulfite solution. This combination enables the antibody to access the epitope site in unfolded analyte protein. The newly developed ELISA recovered 87.1%–106.4% ovalbumin from ovalbumin-incurred model processed foods, thereby demonstrating its applicability as practical egg allergen determination. Furthermore, the comparison of LC-MS/MS and the new ELISA, which targets the amino acid sequence conforming to the LC-MS/MS detection peptide, showed a good agreement. Consequently the harmonization of two methods was demonstrated. The complementary use of the new ELISA and LC-MS analysis can offer a wide range of practical benefits in terms of easiness, cost, accuracy, and efficiency in food allergen analysis. In addition, the new assay is attractive in respect to its easy antigen preparation and predetermined specificity. [Figure not available: see fulltext.].

Original languageEnglish
Pages (from-to)325-335
Number of pages11
JournalAnalytical and Bioanalytical Chemistry
Volume410
Issue number2
DOIs
Publication statusPublished - Jan 1 2018

Fingerprint

Liquid chromatography
Immunoassay
Liquid Chromatography
Allergens
Mass spectrometry
Ovum
Mass Spectrometry
Enzyme-Linked Immunosorbent Assay
Ovalbumin
Processed foods
Allergies
Antigens
Amino Acids
Sulfites
Peptides
Amino Acid Sequence
Sodium Dodecyl Sulfate
Labeling
Epitopes
Assays

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry

Cite this

A harmonized immunoassay with liquid chromatography-mass spectrometry analysis in egg allergen determination. / Nimata, Masaomi; Okada, Hideki; Kurihara, Kei; Sugimoto, Tsukasa; Honjoh, Tsutomu; Kuroda, Kazuhiko; Yano, Takeo; Tachibana, Hirofumi; Shoji, Masahiro.

In: Analytical and Bioanalytical Chemistry, Vol. 410, No. 2, 01.01.2018, p. 325-335.

Research output: Contribution to journalArticle

Nimata, Masaomi ; Okada, Hideki ; Kurihara, Kei ; Sugimoto, Tsukasa ; Honjoh, Tsutomu ; Kuroda, Kazuhiko ; Yano, Takeo ; Tachibana, Hirofumi ; Shoji, Masahiro. / A harmonized immunoassay with liquid chromatography-mass spectrometry analysis in egg allergen determination. In: Analytical and Bioanalytical Chemistry. 2018 ; Vol. 410, No. 2. pp. 325-335.
@article{ba10b1faaee948da80765861a463d3f9,
title = "A harmonized immunoassay with liquid chromatography-mass spectrometry analysis in egg allergen determination",
abstract = "Food allergy is a serious health issue worldwide. Implementing allergen labeling regulations is extremely challenging for regulators, food manufacturers, and analytical kit manufacturers. Here we have developed an “amino acid sequence immunoassay” approach to ELISA. The new ELISA comprises of a monoclonal antibody generated via an analyte specific peptide antigen and sodium lauryl sulfate/sulfite solution. This combination enables the antibody to access the epitope site in unfolded analyte protein. The newly developed ELISA recovered 87.1{\%}–106.4{\%} ovalbumin from ovalbumin-incurred model processed foods, thereby demonstrating its applicability as practical egg allergen determination. Furthermore, the comparison of LC-MS/MS and the new ELISA, which targets the amino acid sequence conforming to the LC-MS/MS detection peptide, showed a good agreement. Consequently the harmonization of two methods was demonstrated. The complementary use of the new ELISA and LC-MS analysis can offer a wide range of practical benefits in terms of easiness, cost, accuracy, and efficiency in food allergen analysis. In addition, the new assay is attractive in respect to its easy antigen preparation and predetermined specificity. [Figure not available: see fulltext.].",
author = "Masaomi Nimata and Hideki Okada and Kei Kurihara and Tsukasa Sugimoto and Tsutomu Honjoh and Kazuhiko Kuroda and Takeo Yano and Hirofumi Tachibana and Masahiro Shoji",
year = "2018",
month = "1",
day = "1",
doi = "10.1007/s00216-017-0721-z",
language = "English",
volume = "410",
pages = "325--335",
journal = "Fresenius Zeitschrift fur Analytische Chemie",
issn = "0016-1152",
publisher = "Springer Verlag",
number = "2",

}

TY - JOUR

T1 - A harmonized immunoassay with liquid chromatography-mass spectrometry analysis in egg allergen determination

AU - Nimata, Masaomi

AU - Okada, Hideki

AU - Kurihara, Kei

AU - Sugimoto, Tsukasa

AU - Honjoh, Tsutomu

AU - Kuroda, Kazuhiko

AU - Yano, Takeo

AU - Tachibana, Hirofumi

AU - Shoji, Masahiro

PY - 2018/1/1

Y1 - 2018/1/1

N2 - Food allergy is a serious health issue worldwide. Implementing allergen labeling regulations is extremely challenging for regulators, food manufacturers, and analytical kit manufacturers. Here we have developed an “amino acid sequence immunoassay” approach to ELISA. The new ELISA comprises of a monoclonal antibody generated via an analyte specific peptide antigen and sodium lauryl sulfate/sulfite solution. This combination enables the antibody to access the epitope site in unfolded analyte protein. The newly developed ELISA recovered 87.1%–106.4% ovalbumin from ovalbumin-incurred model processed foods, thereby demonstrating its applicability as practical egg allergen determination. Furthermore, the comparison of LC-MS/MS and the new ELISA, which targets the amino acid sequence conforming to the LC-MS/MS detection peptide, showed a good agreement. Consequently the harmonization of two methods was demonstrated. The complementary use of the new ELISA and LC-MS analysis can offer a wide range of practical benefits in terms of easiness, cost, accuracy, and efficiency in food allergen analysis. In addition, the new assay is attractive in respect to its easy antigen preparation and predetermined specificity. [Figure not available: see fulltext.].

AB - Food allergy is a serious health issue worldwide. Implementing allergen labeling regulations is extremely challenging for regulators, food manufacturers, and analytical kit manufacturers. Here we have developed an “amino acid sequence immunoassay” approach to ELISA. The new ELISA comprises of a monoclonal antibody generated via an analyte specific peptide antigen and sodium lauryl sulfate/sulfite solution. This combination enables the antibody to access the epitope site in unfolded analyte protein. The newly developed ELISA recovered 87.1%–106.4% ovalbumin from ovalbumin-incurred model processed foods, thereby demonstrating its applicability as practical egg allergen determination. Furthermore, the comparison of LC-MS/MS and the new ELISA, which targets the amino acid sequence conforming to the LC-MS/MS detection peptide, showed a good agreement. Consequently the harmonization of two methods was demonstrated. The complementary use of the new ELISA and LC-MS analysis can offer a wide range of practical benefits in terms of easiness, cost, accuracy, and efficiency in food allergen analysis. In addition, the new assay is attractive in respect to its easy antigen preparation and predetermined specificity. [Figure not available: see fulltext.].

UR - http://www.scopus.com/inward/record.url?scp=85033732427&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85033732427&partnerID=8YFLogxK

U2 - 10.1007/s00216-017-0721-z

DO - 10.1007/s00216-017-0721-z

M3 - Article

VL - 410

SP - 325

EP - 335

JO - Fresenius Zeitschrift fur Analytische Chemie

JF - Fresenius Zeitschrift fur Analytische Chemie

SN - 0016-1152

IS - 2

ER -