TY - JOUR
T1 - A high-density STS map based on a single contig of YAC and P1 clones in the chromosome 8p12-p21 region
AU - Mitsuda, Noriaki
AU - Nakura, Jun
AU - Ye, Lin
AU - Zhao, Yi
AU - Fujioka, Yoshihiko
AU - Takahashi-Fujii, Asako
AU - Ishino, Yoshizumi
AU - Kato, Ikunoshin
AU - Hashimoto, Katsuyuki
AU - Ogihara, Toshio
AU - Miki, Tetsuro
N1 - Funding Information:
The authors are very grateful to Dr. Takashi Imai and Dr. Yusuke Nakamura for providing the St. Louis and CEPH YAC libraries. We are also grateful to Dr. Fumihiko Matsuda and Dr. Tasuku Honjo for providing the Du Pont±Merck Pharmaceutical Co. P1 phage library. This work was supported by a Grant-in-Aid for Creative Basic Research (Human Genome Program) from the Ministry of Education, Science and Culture of Japan (to T.M.) and a grant from the Research Project on Health and Aging (to J.N.).
PY - 1997/4/1
Y1 - 1997/4/1
N2 - We have constructed a yeast artificial chromosome (YAC) and P1 contig in the 8p12-p21 region. The contig comprises 16 overlapping YAC clones and 44 overlapping P1 clones. Twelve dinucleotide-repeat polymorphic sequence- tagged site (STS) markers that were previously isolated mainly from these YAC and P1 clones were genetically mapped. A total of 46 nonpolymorphic STS markers were newly established mainly from the YAC and P1 clone end fragments, and 28 of the 46 nonpolymorphic STSs, as well as the 12 polymorphic STSs, were also mapped physically onto the contig based on STS content analysis of YAC pools and of the P1 and YAC clones. As a result, the YAC and P1 clones were assembled into a single contig covering a minimum of 1.5 Mb physically and 2.8 cM genetically with 12 polymorphic and 28 nonpolymorphic STSs within the 8p12-p21 region. Average STS spacing in the contig was estimated to be 40 kb/STS. In addition, further characterization of the contig suggested that this contig includes a region where genetic recombination occurs frequently. Thus, the resulting cloned region, together with densely mapped STS markers on the contig, should help to promote our understanding of this region.
AB - We have constructed a yeast artificial chromosome (YAC) and P1 contig in the 8p12-p21 region. The contig comprises 16 overlapping YAC clones and 44 overlapping P1 clones. Twelve dinucleotide-repeat polymorphic sequence- tagged site (STS) markers that were previously isolated mainly from these YAC and P1 clones were genetically mapped. A total of 46 nonpolymorphic STS markers were newly established mainly from the YAC and P1 clone end fragments, and 28 of the 46 nonpolymorphic STSs, as well as the 12 polymorphic STSs, were also mapped physically onto the contig based on STS content analysis of YAC pools and of the P1 and YAC clones. As a result, the YAC and P1 clones were assembled into a single contig covering a minimum of 1.5 Mb physically and 2.8 cM genetically with 12 polymorphic and 28 nonpolymorphic STSs within the 8p12-p21 region. Average STS spacing in the contig was estimated to be 40 kb/STS. In addition, further characterization of the contig suggested that this contig includes a region where genetic recombination occurs frequently. Thus, the resulting cloned region, together with densely mapped STS markers on the contig, should help to promote our understanding of this region.
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U2 - 10.1006/geno.1997.4619
DO - 10.1006/geno.1997.4619
M3 - Article
C2 - 9126481
AN - SCOPUS:0031127214
SN - 0888-7543
VL - 41
SP - 49
EP - 55
JO - Genomics
JF - Genomics
IS - 1
ER -