A high phosphorus diet affects lipid metabolism in rat liver: A DNA microarray analysis

Sunwoo Chun, Takeshi Bamba, Tatsuya Suyama, Tomoko Ishijima, Eiichiro Fukusaki, Keiko Abe, Yuji Nakai

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

A high phosphorus (HP) diet causes disorders of renal function, bone metabolism, and vascular function. We previously demonstrated that DNA microarray analysis is an appropriate method to comprehensively evaluate the effects of a HP diet on kidney dysfunction such as calcification, fibrillization, and inflammation. We reported that type IIb sodium-dependent phosphate transporter is significantly up-regulated in this context. In the present study, we performed DNA microarray analysis to investigate the effects of a HP diet on the liver, which plays a pivotal role in energy metabolism. DNA microarray analysis was performed with total RNA isolated from the livers of rats fed a control diet (containing 0.3% phosphorus) or a HP diet (containing 1.2% phosphorus). Gene Ontology analysis of differentially expressed genes (DEGs) revealed that the HP diet induced down-regulation of genes involved in hepatic amino acid catabolism and lipogenesis, while genes related to fatty acid β-oxidation process were up-regulated. Although genes related to fatty acid biosynthesis were downregulated in HP diet-fed rats, genes important for the elongation and desaturation reactions of omega-3 and -6 fatty acids were up-regulated. Concentrations of hepatic arachidonic acid and eicosapentaenoic acid were increased in HP diet-fed rats. These essential fatty acids activate peroxisome proliferator-activated receptor alpha (PPARα), a transcription factor for fatty acid β-oxidation. Evaluation of the upstream regulators of DEGs using Ingenuity Pathway Analysis indicated that PPARα was activated in the livers of HP diet-fed rats. Furthermore, the serum concentration of fibroblast growth factor 21, a hormone secreted from the liver that promotes fatty acid utilization in adipose tissue as a PPARα target gene, was higher (p = 0.054) in HP diet-fed rats than in control diet-fed rats. These data suggest that a HP diet enhances energy expenditure through the utilization of free fatty acids released via lipolysis of white adipose tissue.

Original languageEnglish
Article numbere0155386
JournalPLoS One
Volume11
Issue number5
DOIs
Publication statusPublished - May 1 2016

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Microarray Analysis
Microarrays
Nutrition
Oligonucleotide Array Sequence Analysis
Lipid Metabolism
lipid metabolism
Liver
Phosphorus
Rats
Diet
phosphorus
liver
DNA
rats
Genes
diet
Fatty Acids
PPAR alpha
genes
beta oxidation

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Chun, S., Bamba, T., Suyama, T., Ishijima, T., Fukusaki, E., Abe, K., & Nakai, Y. (2016). A high phosphorus diet affects lipid metabolism in rat liver: A DNA microarray analysis. PLoS One, 11(5), [e0155386]. https://doi.org/10.1371/journal.pone.0155386

A high phosphorus diet affects lipid metabolism in rat liver : A DNA microarray analysis. / Chun, Sunwoo; Bamba, Takeshi; Suyama, Tatsuya; Ishijima, Tomoko; Fukusaki, Eiichiro; Abe, Keiko; Nakai, Yuji.

In: PLoS One, Vol. 11, No. 5, e0155386, 01.05.2016.

Research output: Contribution to journalArticle

Chun, S, Bamba, T, Suyama, T, Ishijima, T, Fukusaki, E, Abe, K & Nakai, Y 2016, 'A high phosphorus diet affects lipid metabolism in rat liver: A DNA microarray analysis', PLoS One, vol. 11, no. 5, e0155386. https://doi.org/10.1371/journal.pone.0155386
Chun, Sunwoo ; Bamba, Takeshi ; Suyama, Tatsuya ; Ishijima, Tomoko ; Fukusaki, Eiichiro ; Abe, Keiko ; Nakai, Yuji. / A high phosphorus diet affects lipid metabolism in rat liver : A DNA microarray analysis. In: PLoS One. 2016 ; Vol. 11, No. 5.
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