A high-sensitivity cyclodextrin-microchip electrophoresis simultaneous analysis of gabapentin and pregabalin

A. M. Zeid, J. Nasr, N. Kaji, F. F. Belal, M. I. Walash, Y. Baba

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We report a highly-sensitive microchip electrophoretic method for the simultaneous analysis of gabapentin (GPN) and pregabalin (PGN). Analyses and separation of both compounds were achieved in a PMMA microchip after labelling with 4-Fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F). The separation of the labelled compounds was improved by addition of β-cyclodextrin (β-CD). Moreover, methylcellulose polymer was added to prevent analytes' adsorption on the microchannel walls. The sensitivity of the method was improved by stacking to quantify as low as 0.06 nM of each drug with detection limits of 12 pM.

Original languageEnglish
Title of host publication20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
PublisherChemical and Biological Microsystems Society
Pages1457-1458
Number of pages2
ISBN (Electronic)9780979806490
Publication statusPublished - Jan 1 2016
Externally publishedYes
Event20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 - Dublin, Ireland
Duration: Oct 9 2016Oct 13 2016

Publication series

Name20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016

Other

Other20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
CountryIreland
CityDublin
Period10/9/1610/13/16

All Science Journal Classification (ASJC) codes

  • Control and Systems Engineering

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  • Cite this

    Zeid, A. M., Nasr, J., Kaji, N., Belal, F. F., Walash, M. I., & Baba, Y. (2016). A high-sensitivity cyclodextrin-microchip electrophoresis simultaneous analysis of gabapentin and pregabalin. In 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 (pp. 1457-1458). (20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016). Chemical and Biological Microsystems Society.