A highly efficient siRNA carrier of PBLG modified hyperbranched PEI

Jie Chen, Huayu Tian, Zhaopei Guo, Jialiang Xia, Arihiro Kano, Atsushi Maruyama, Xiabin Jing, Xuesi Chen

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

In search for effective non-viral gene vectors for the delivery of siRNA, a copolymer was designed and synthesized by grafting hydrophobic poly(γ-benzyl L-glutamate) segment (PBLG) to hyperbranched polyethylenimine (PEI-PBLG). PEI-PBLG could efficiently deliver siRNA to cells to silence the targeted gene. Markedly, PEI-PBLG caused lower cytotoxicity in comparison to unmodified PEI. The siRNA complexed with PEI-PBLG showed a remarkable knockdown (75.23% relative to untreated cells, without changing the medium after 6 h of incubation) of the targeted luciferase gene in stable expressing luciferase CT26 cells while the Lipofectamine ™2000 and unmodified PEI could only achieve knockdown rates of 57.92% and 15.31%, respectively. The siRNA complexed with PEI-PBLG also demonstrated that it had greater gene silencing ability than unmodified PEI and Lipofectamin™2000 in both 4T1 cells stably transfected with the luciferase gene and HeLa cells transiently transfected with the luciferase gene. The internalization efficiency of carrier/ Alexa647-labeled siRNA was quantified using flow cytometry. PEI-PBLG/Alexa647-labeled siRNA showed internalization efficiency of 52.67% while PEI and Lipofectamine™2000 demonstrated 27.23% and 37.91%, respectively. Confocal laser scanning microscopy (CLSM) assay also indicated that PEIPBLG induced higher cell uptake efficiency than other commercial reagents. PEI-PBLG was shown to be a promising siRNA carrier with potential application in cancer therapy.

Original languageEnglish
Pages (from-to)1247-1253
Number of pages7
JournalMacromolecular Bioscience
Volume9
Issue number12
DOIs
Publication statusPublished - Dec 8 2009

Fingerprint

Polyetherimides
Small Interfering RNA
Genes
Luciferases
Polyethyleneimine
Flow cytometry
Gene Silencing
Cytotoxicity
polyethylenimine-poly(gamma-benzylglutamate)
HeLa Cells
Confocal Microscopy
Assays
Flow Cytometry
Microscopic examination
Copolymers
Scanning
Lasers

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Biomaterials
  • Polymers and Plastics
  • Materials Chemistry

Cite this

Chen, J., Tian, H., Guo, Z., Xia, J., Kano, A., Maruyama, A., ... Chen, X. (2009). A highly efficient siRNA carrier of PBLG modified hyperbranched PEI. Macromolecular Bioscience, 9(12), 1247-1253. https://doi.org/10.1002/mabi.200900249

A highly efficient siRNA carrier of PBLG modified hyperbranched PEI. / Chen, Jie; Tian, Huayu; Guo, Zhaopei; Xia, Jialiang; Kano, Arihiro; Maruyama, Atsushi; Jing, Xiabin; Chen, Xuesi.

In: Macromolecular Bioscience, Vol. 9, No. 12, 08.12.2009, p. 1247-1253.

Research output: Contribution to journalArticle

Chen, J, Tian, H, Guo, Z, Xia, J, Kano, A, Maruyama, A, Jing, X & Chen, X 2009, 'A highly efficient siRNA carrier of PBLG modified hyperbranched PEI', Macromolecular Bioscience, vol. 9, no. 12, pp. 1247-1253. https://doi.org/10.1002/mabi.200900249
Chen, Jie ; Tian, Huayu ; Guo, Zhaopei ; Xia, Jialiang ; Kano, Arihiro ; Maruyama, Atsushi ; Jing, Xiabin ; Chen, Xuesi. / A highly efficient siRNA carrier of PBLG modified hyperbranched PEI. In: Macromolecular Bioscience. 2009 ; Vol. 9, No. 12. pp. 1247-1253.
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