A hydrophilic polymer grafted with a histone tail peptide as an artificial gene regulator

Shujiro Shiosaki, Masanori Kuramoto, Riki Toita, Takeshi Mori, Takuro Niidome, Yoshiki Katayama

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

In chromatin, gene transcription is regulated through posttranslational modifications on the histone N-terminal tail sequences, typically an acetyl group modification on lysine residues. To realize a simple model of the gene regulation of chromatin, we designed a hydrophilic polymer grafted with histone H3 tail peptides. The polyplex formed from the polymer and DNA suppressed the gene expression effectively although the polyplex was weaker than the polyplex of poly-l-lysine and DNA. This weaker polyplex afforded the acetylation of the lysine residue of the grafted peptides by histone acetyltransferase. Subsequently, the gene expression was activated due to the relaxation of the polyplex which was brought by a cationic charge decrease in the grafted peptides. This molecular system is the first functional model of the gene regulation of the chromatin.

Original languageEnglish
Pages (from-to)4101-4105
Number of pages5
JournalBioorganic and Medicinal Chemistry
Volume19
Issue number13
DOIs
Publication statusPublished - Jul 1 2011

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Pharmaceutical Science
  • Drug Discovery
  • Clinical Biochemistry
  • Organic Chemistry

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