A leech capable of surviving exposure to extremely low temperatures

Dai Suzuki, Tomoko Miyamoto, Takahiro Kikawada, Manabu Watanabe, Toru Suzuki

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

It is widely considered that most organisms cannot survive prolonged exposure to temperatures below 0uC, primarily because of the damage caused by the water in cells as it freezes. However, some organisms are capable of surviving extreme variations in environmental conditions. In the case of temperature, the ability to survive subzero temperatures is referred to as cryobiosis. We show that the ozobranchid leech, Ozobranchus jantseanus, a parasite of freshwater turtles, has a surprisingly high tolerance to freezing and thawing. This finding is particularly interesting because the leach can survive these temperatures without any acclimation period or pretreatment. Specifically, the leech survived exposure to super-low temperatures by storage in liquid nitrogen (-196°C) for 24 hours, as well as long-term storage at temperatures as low as -90°C for up to 32 months. The leech was also capable of enduring repeated freeze-thaw cycles in the temperature range 20°C to -100°C and then back to 20°C. The results demonstrated that the novel cryotolerance mechanisms employed by O. jantseanus enable the leech to withstand a wider range of temperatures than those reported previously for cryobiotic organisms. We anticipate that the mechanism for the observed tolerance to freezing and thawing in O. jantseanus will prove useful for future studies of cryopreservation.

Original languageEnglish
Article numbere86807
JournalPloS one
Volume9
Issue number1
DOIs
Publication statusPublished - Jan 22 2014

Fingerprint

Leeches
Hirudinea
Temperature
temperature
Thawing
Freezing
thawing
organisms
freezing
Turtles
freeze-thaw cycles
Acclimatization
Cryopreservation
Liquid nitrogen
Fresh Water
cryopreservation
turtles
acclimation
Parasites
Nitrogen

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

Cite this

Suzuki, D., Miyamoto, T., Kikawada, T., Watanabe, M., & Suzuki, T. (2014). A leech capable of surviving exposure to extremely low temperatures. PloS one, 9(1), [e86807]. https://doi.org/10.1371/journal.pone.0086807

A leech capable of surviving exposure to extremely low temperatures. / Suzuki, Dai; Miyamoto, Tomoko; Kikawada, Takahiro; Watanabe, Manabu; Suzuki, Toru.

In: PloS one, Vol. 9, No. 1, e86807, 22.01.2014.

Research output: Contribution to journalArticle

Suzuki, D, Miyamoto, T, Kikawada, T, Watanabe, M & Suzuki, T 2014, 'A leech capable of surviving exposure to extremely low temperatures', PloS one, vol. 9, no. 1, e86807. https://doi.org/10.1371/journal.pone.0086807
Suzuki D, Miyamoto T, Kikawada T, Watanabe M, Suzuki T. A leech capable of surviving exposure to extremely low temperatures. PloS one. 2014 Jan 22;9(1). e86807. https://doi.org/10.1371/journal.pone.0086807
Suzuki, Dai ; Miyamoto, Tomoko ; Kikawada, Takahiro ; Watanabe, Manabu ; Suzuki, Toru. / A leech capable of surviving exposure to extremely low temperatures. In: PloS one. 2014 ; Vol. 9, No. 1.
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