A luminol chemiluminescence method for sensing histidine and lysine using enzyme reactions

Akimitsu Kugimiya, Rie Fukada, Daiki Funamoto

    Research output: Contribution to journalArticlepeer-review

    16 Citations (Scopus)

    Abstract

    The analysis of free amino acids in urine and plasma is useful for estimating disease status in clinical diagnoses. Changes in the concentration of free amino acids in foods are also useful markers of freshness, nutrition, and taste. In this study, the specific interaction between aminoacyl-tRNA synthetase (aaRS) and its corresponding amino acid was used to measure amino acid concentrations. Pyrophosphate released by the amino acid-aaRS binding reaction was detected by luminol chemiluminescence; the method provided selective quantitation of 1.0-30 μM histidine and 1.0-60 μM lysine.

    Original languageEnglish
    Pages (from-to)22-26
    Number of pages5
    JournalAnalytical Biochemistry
    Volume443
    Issue number1
    DOIs
    Publication statusPublished - Jan 1 2013

    All Science Journal Classification (ASJC) codes

    • Biophysics
    • Biochemistry
    • Molecular Biology
    • Cell Biology

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