A major allogenic leukocyte antigen in the agnathan hagfish

Hiroyuki Takaba, Takeshi Imai, Shoji Miki, Yasuyuki Morishita, Akihiro Miyashita, Naoko Ishikawa, Hirofumi Nishizumi, Hitoshi Sakano

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

All vertebrates, from jawless fish to mammals, possess adaptive immune systems that can detect and inactivate non-self-antigens through a vast repertoire of antigen receptors. Unlike jawed vertebrates, the hagfish utilizes variable lymphocyte receptors (VLRs) that are unrelated to immunoglobulin molecules but are diversified by copy-choice gene conversion mechanism. Here, we report that hagfish VLRs react with allogenic leukocyte antigens but not with self-antigens. We found that a highly polymorphic membrane protein, NICIR3, is recognized by VLRs as an allogenic leukocyte antigen (ALA). In a serological cross-reactivity test, a close correlation was observed between the amino acid differences in the protein sequences and the VLR cross-reactivities. This leukocyte antigen was predominantly expressed in phagocytic leukocytes, where it was associated with phagocytosed protein antigens. These findings suggest that a polymorphic leukocyte antigen, NICIR3/ALA, plays a pivotal role in jawless vertebrate adaptive immunity.

Original languageEnglish
Article number01716
JournalScientific reports
Volume3
DOIs
Publication statusPublished - Apr 24 2013
Externally publishedYes

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Hagfishes
HLA Antigens
Lymphocytes
Vertebrates
Antigens
Gene Conversion
Antigen Receptors
Autoantigens
Adaptive Immunity
Phagocytosis
Immunoglobulins
Mammals
Immune System
Membrane Proteins
Fishes
Proteins
Leukocytes
Amino Acids

All Science Journal Classification (ASJC) codes

  • General

Cite this

Takaba, H., Imai, T., Miki, S., Morishita, Y., Miyashita, A., Ishikawa, N., ... Sakano, H. (2013). A major allogenic leukocyte antigen in the agnathan hagfish. Scientific reports, 3, [01716]. https://doi.org/10.1038/srep01716

A major allogenic leukocyte antigen in the agnathan hagfish. / Takaba, Hiroyuki; Imai, Takeshi; Miki, Shoji; Morishita, Yasuyuki; Miyashita, Akihiro; Ishikawa, Naoko; Nishizumi, Hirofumi; Sakano, Hitoshi.

In: Scientific reports, Vol. 3, 01716, 24.04.2013.

Research output: Contribution to journalArticle

Takaba, H, Imai, T, Miki, S, Morishita, Y, Miyashita, A, Ishikawa, N, Nishizumi, H & Sakano, H 2013, 'A major allogenic leukocyte antigen in the agnathan hagfish', Scientific reports, vol. 3, 01716. https://doi.org/10.1038/srep01716
Takaba H, Imai T, Miki S, Morishita Y, Miyashita A, Ishikawa N et al. A major allogenic leukocyte antigen in the agnathan hagfish. Scientific reports. 2013 Apr 24;3. 01716. https://doi.org/10.1038/srep01716
Takaba, Hiroyuki ; Imai, Takeshi ; Miki, Shoji ; Morishita, Yasuyuki ; Miyashita, Akihiro ; Ishikawa, Naoko ; Nishizumi, Hirofumi ; Sakano, Hitoshi. / A major allogenic leukocyte antigen in the agnathan hagfish. In: Scientific reports. 2013 ; Vol. 3.
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