A measuring method for occupancy of immune checkpoint inhibitors in the cell surface

Toyoshi Yanagihara, Kentaro Tanaka, Koichiro Matsumoto

Research output: Contribution to journalArticle

Abstract

Monoclonal antibodies, including immune-checkpoint inhibitors, are becoming popular in treatments of many cancers and connective tissue diseases. However, little is known about how long the antibodies combine with antigens on targeted cells or how this duration of binding associates with therapeutic efficacy or potential adverse events. Here, we show the principle and the results of a feasible method for measuring the antibodies’ occupancy on the targeted cells using two different detecting antibodies in conjunction with different fluorochromes. Nivolumab occupancy was measured using two detecting antibodies, MIH4 and EH12.2, which are commercially available in vitro (programmed cell death-1 [PD-1] expressing the cell line MIT9 and human T cells) and in T cells from patients treated with nivolumab. Our method has potential for use as a simple and feasible monitoring system in the clinical setting.

Original languageEnglish
Pages (from-to)213-217
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume527
Issue number1
DOIs
Publication statusPublished - Jun 18 2020

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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