A mechanism of transcriptional regulation of the CSF-1 gene by interferon-gamma

Daisuke Tsuchimoto, Arinobu Tojo, Shigetaka Asano

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

Interferon (IFN)-gamma exerts multiple functions including antiviral, anti-proliferative and immunomodulatory activities, which are mediated through the JAK-STAT pathway. We observed that IFN-gamma significantly increases the production of colony stimulating factor-1 (CSF-1) by a human lung carcinoma cell line, A549. To gain insight into this mechanism, we determined the unknown nucleotide sequences of 5′-flanking region of human CSF-1 gene. About 60 bases upstream of the transcription start site of the CSF-1 gene contains a possible gamma interferon activated site (GAS), TTCCCATAA. The promoter assay and the electrophoretic mobility gel shift assay revealed that IFN-gamma stimulates transcription of the CSF-1 gene through this sequence, which binds STAT1.

Original languageEnglish
Pages (from-to)397-405
Number of pages9
JournalImmunological Investigations
Volume33
Issue number4
DOIs
Publication statusPublished - 2004

All Science Journal Classification (ASJC) codes

  • Immunology

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