Abstract
Interferon (IFN)-gamma exerts multiple functions including antiviral, anti-proliferative and immunomodulatory activities, which are mediated through the JAK-STAT pathway. We observed that IFN-gamma significantly increases the production of colony stimulating factor-1 (CSF-1) by a human lung carcinoma cell line, A549. To gain insight into this mechanism, we determined the unknown nucleotide sequences of 5′-flanking region of human CSF-1 gene. About 60 bases upstream of the transcription start site of the CSF-1 gene contains a possible gamma interferon activated site (GAS), TTCCCATAA. The promoter assay and the electrophoretic mobility gel shift assay revealed that IFN-gamma stimulates transcription of the CSF-1 gene through this sequence, which binds STAT1.
| Original language | English |
|---|---|
| Pages (from-to) | 397-405 |
| Number of pages | 9 |
| Journal | Immunological Investigations |
| Volume | 33 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - Dec 15 2004 |
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All Science Journal Classification (ASJC) codes
- Immunology
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A mechanism of transcriptional regulation of the CSF-1 gene by interferon-gamma. / Tsuchimoto, Daisuke; Tojo, Arinobu; Asano, Shigetaka.
In: Immunological Investigations, Vol. 33, No. 4, 15.12.2004, p. 397-405.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A mechanism of transcriptional regulation of the CSF-1 gene by interferon-gamma
AU - Tsuchimoto, Daisuke
AU - Tojo, Arinobu
AU - Asano, Shigetaka
PY - 2004/12/15
Y1 - 2004/12/15
N2 - Interferon (IFN)-gamma exerts multiple functions including antiviral, anti-proliferative and immunomodulatory activities, which are mediated through the JAK-STAT pathway. We observed that IFN-gamma significantly increases the production of colony stimulating factor-1 (CSF-1) by a human lung carcinoma cell line, A549. To gain insight into this mechanism, we determined the unknown nucleotide sequences of 5′-flanking region of human CSF-1 gene. About 60 bases upstream of the transcription start site of the CSF-1 gene contains a possible gamma interferon activated site (GAS), TTCCCATAA. The promoter assay and the electrophoretic mobility gel shift assay revealed that IFN-gamma stimulates transcription of the CSF-1 gene through this sequence, which binds STAT1.
AB - Interferon (IFN)-gamma exerts multiple functions including antiviral, anti-proliferative and immunomodulatory activities, which are mediated through the JAK-STAT pathway. We observed that IFN-gamma significantly increases the production of colony stimulating factor-1 (CSF-1) by a human lung carcinoma cell line, A549. To gain insight into this mechanism, we determined the unknown nucleotide sequences of 5′-flanking region of human CSF-1 gene. About 60 bases upstream of the transcription start site of the CSF-1 gene contains a possible gamma interferon activated site (GAS), TTCCCATAA. The promoter assay and the electrophoretic mobility gel shift assay revealed that IFN-gamma stimulates transcription of the CSF-1 gene through this sequence, which binds STAT1.
UR - http://www.scopus.com/inward/record.url?scp=9744282598&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=9744282598&partnerID=8YFLogxK
U2 - 10.1081/IMM-200038662
DO - 10.1081/IMM-200038662
M3 - Article
C2 - 15624698
AN - SCOPUS:9744282598
VL - 33
SP - 397
EP - 405
JO - Immunological Investigations
JF - Immunological Investigations
SN - 0882-0139
IS - 4
ER -