TY - JOUR
T1 - A mechanism of transcriptional regulation of the CSF-1 gene by interferon-gamma
AU - Tsuchimoto, Daisuke
AU - Tojo, Arinobu
AU - Asano, Shigetaka
PY - 2004
Y1 - 2004
N2 - Interferon (IFN)-gamma exerts multiple functions including antiviral, anti-proliferative and immunomodulatory activities, which are mediated through the JAK-STAT pathway. We observed that IFN-gamma significantly increases the production of colony stimulating factor-1 (CSF-1) by a human lung carcinoma cell line, A549. To gain insight into this mechanism, we determined the unknown nucleotide sequences of 5′-flanking region of human CSF-1 gene. About 60 bases upstream of the transcription start site of the CSF-1 gene contains a possible gamma interferon activated site (GAS), TTCCCATAA. The promoter assay and the electrophoretic mobility gel shift assay revealed that IFN-gamma stimulates transcription of the CSF-1 gene through this sequence, which binds STAT1.
AB - Interferon (IFN)-gamma exerts multiple functions including antiviral, anti-proliferative and immunomodulatory activities, which are mediated through the JAK-STAT pathway. We observed that IFN-gamma significantly increases the production of colony stimulating factor-1 (CSF-1) by a human lung carcinoma cell line, A549. To gain insight into this mechanism, we determined the unknown nucleotide sequences of 5′-flanking region of human CSF-1 gene. About 60 bases upstream of the transcription start site of the CSF-1 gene contains a possible gamma interferon activated site (GAS), TTCCCATAA. The promoter assay and the electrophoretic mobility gel shift assay revealed that IFN-gamma stimulates transcription of the CSF-1 gene through this sequence, which binds STAT1.
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U2 - 10.1081/IMM-200038662
DO - 10.1081/IMM-200038662
M3 - Article
C2 - 15624698
AN - SCOPUS:9744282598
VL - 33
SP - 397
EP - 405
JO - Immunological Investigations
JF - Immunological Investigations
SN - 0882-0139
IS - 4
ER -