A method for in-situ targeting and harvesting of cultured cells under a microscope

Hiroshi Takamatsu, Satoru Uchida, Takehisa Matsuda

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

A new technique was developed to select and harvest cultured cells that are targeted under a microscope. It utilizes a thermo-responsive dish on which cells are adhesive at temperatures above ∼33°C and are separated from the surface only by cooling. The principle of the technique is to culture cells on a thermo-responsive dish cool locally beneath a cell that are observed under a microscope and harvest detached cells that lost their extracellular matrix using a micropipette We developed a thermo-responsive dish by coating polymers, a mixture of Poly(N-isopropylacrylamide) (PNIPAAm) and PNIPAAm-grafted gelatin. A device was also developed to cool only a small part of the bottom of the dish beneath a cell that was targeted under a microscope. It was demonstrated that a cell or two cells that adhered each other were detached from the surtace and harvested by a micropipette within about thirty seconds.

Original languageEnglish
Title of host publication2006 IEEE International Symposium on Micro-Nano Mechanical and Human Science, MHS
DOIs
Publication statusPublished - Dec 1 2006
Event2006 IEEE International Symposium on Micro-Nano Mechanical and Human Science, MHS - Nagoya, Japan
Duration: Nov 5 2006Nov 8 2006

Publication series

Name2006 IEEE International Symposium on Micro-Nano Mechanical and Human Science, MHS

Other

Other2006 IEEE International Symposium on Micro-Nano Mechanical and Human Science, MHS
CountryJapan
CityNagoya
Period11/5/0611/8/06

All Science Journal Classification (ASJC) codes

  • Computational Mechanics
  • Control and Systems Engineering
  • Human Factors and Ergonomics

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