A method for measuring the three-dimensional refractive-index distribution of single cells using proximal two-beam optical tweezers and a phase-shifting mach-zehnder interferometer

Toshiki Yasokawa, Ichirou Ishimaru, Masahiro Kondo, Shigeki Kuriyama, Tsutomu Masaki, Kaoru Takegawa, Naotaka Tanaka

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

This paper describes a method for measuring the three-dimensional (3D) refractive-index distribution in a single cell. The method can be used to observe the distribution of cell components without fluorescence staining. The two-dimensional optical path length distributions from multiple directions are obtained by non-contact rotation of the cell. These optical path lengths are converted into the line integrals of the refractive index, and the 3D refractive-index distribution is reconstructed by means of computed tomography. The refractive-index distribution in a breast cancer cell can be measured using a phase-shifting Mach-Zehnder interferometer in conjunction with proximal two-beam optical tweezers.

Original languageEnglish
Pages (from-to)161-164
Number of pages4
JournalOptical Review
Volume14
Issue number4
DOIs
Publication statusPublished - Jul 1 2007

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Mach-Zehnder interferometers
refractivity
cells
optical paths
staining
breast
tomography
cancer
fluorescence

All Science Journal Classification (ASJC) codes

  • Atomic and Molecular Physics, and Optics

Cite this

A method for measuring the three-dimensional refractive-index distribution of single cells using proximal two-beam optical tweezers and a phase-shifting mach-zehnder interferometer. / Yasokawa, Toshiki; Ishimaru, Ichirou; Kondo, Masahiro; Kuriyama, Shigeki; Masaki, Tsutomu; Takegawa, Kaoru; Tanaka, Naotaka.

In: Optical Review, Vol. 14, No. 4, 01.07.2007, p. 161-164.

Research output: Contribution to journalArticle

Yasokawa, Toshiki ; Ishimaru, Ichirou ; Kondo, Masahiro ; Kuriyama, Shigeki ; Masaki, Tsutomu ; Takegawa, Kaoru ; Tanaka, Naotaka. / A method for measuring the three-dimensional refractive-index distribution of single cells using proximal two-beam optical tweezers and a phase-shifting mach-zehnder interferometer. In: Optical Review. 2007 ; Vol. 14, No. 4. pp. 161-164.
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