TY - JOUR
T1 - A method to determine the duration of the eclipse phase for in vitro infection with a highly pathogenic SHIV strain
AU - Kakizoe, Yusuke
AU - Nakaoka, Shinji
AU - Beauchemin, Catherine A.A.
AU - Morita, Satoru
AU - Mori, Hiromi
AU - Igarashi, Tatsuhiko
AU - Aihara, Kazuyuki
AU - Miura, Tomoyuki
AU - Iwami, Shingo
N1 - Funding Information:
We thank Yoh Iwasa, Kei Sato, Hisashi Inaba, Yasuhiro Takeuchi, Yoshihisa Morita and Hiroshi Nishiura for many helpful comments on this manuscript. This research was supported in part by the Kyushu University Fund (to Y.K.), Grants-in-Aid for Young Scientists B25871132 (to S.N.) and B25800092 (to S.I.) from the Japan Society for the Promotion of Science (JSPS), the Commissioned Research program of the Ministry of Health, Labour and Welfare, Japan (to S.N., K.A. and S.I. H26-ShinkoJitsuyoka-General-016), Kyushu University Short-term Young Scholar Exchange Program, which is based on Japanese Ministry of Education, Culture, Sports, Science and Technology’s grant The Program for Promoting the Enhancement of Research Universities (to C.A.A.B. and S.I.), Discovery Grant from the Natural Sciences and Engineering Research Council of Canada (to C.A.A.B.), Early Researcher Award (to C.A.A.B) from the Ministry of Research and Innovation of the Government of Ontario, JST CREST program (to S.M., and S.I.), the Aihara Innovative Mathematical Modeling Project, JSPS, through the “Funding Program for World-Leading Innovative R & D on Science and Technology (FIRST Program)”, initiated by Council for Science and Technology Policy (to K.A. and S.I.), JST PRESTO program (to S.I.), JSPS KAKENHI Grant Number 10192783 (to S.I.), with additional funding from the Inamori Foundation (to S.I.).
PY - 2015/5/21
Y1 - 2015/5/21
N2 - The time elapsed between successful cell infection and the start of virus production is called the eclipse phase. Its duration is specific to each virus strain and, along with an effective virus production rate, plays a key role in infection kinetics. How the eclipse phase varies amongst cells infected with the same virus strain and therefore how best to mathematically represent its duration is not clear. Most mathematical models either neglect this phase or assume it is exponentially distributed, such that at least some if not all cells can produce virus immediately upon infection. Biologically, this is unrealistic (one must allow for the translation, transcription, export, etc. to take place), but could be appropriate if the duration of the eclipse phase is negligible on the time-scale of the infection. If it is not, however, ignoring this delay affects the accuracy of the mathematical model, its parameter estimates, and predictions. Here, we introduce a new approach, consisting in a carefully designed experiment and simple analytical expressions, to determine the duration and distribution of the eclipse phase in vitro. We find that the eclipse phase of SHIV-KS661 lasts on average one day and is consistent with an Erlang distribution.
AB - The time elapsed between successful cell infection and the start of virus production is called the eclipse phase. Its duration is specific to each virus strain and, along with an effective virus production rate, plays a key role in infection kinetics. How the eclipse phase varies amongst cells infected with the same virus strain and therefore how best to mathematically represent its duration is not clear. Most mathematical models either neglect this phase or assume it is exponentially distributed, such that at least some if not all cells can produce virus immediately upon infection. Biologically, this is unrealistic (one must allow for the translation, transcription, export, etc. to take place), but could be appropriate if the duration of the eclipse phase is negligible on the time-scale of the infection. If it is not, however, ignoring this delay affects the accuracy of the mathematical model, its parameter estimates, and predictions. Here, we introduce a new approach, consisting in a carefully designed experiment and simple analytical expressions, to determine the duration and distribution of the eclipse phase in vitro. We find that the eclipse phase of SHIV-KS661 lasts on average one day and is consistent with an Erlang distribution.
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U2 - 10.1038/srep10371
DO - 10.1038/srep10371
M3 - Article
C2 - 25996439
AN - SCOPUS:84930216589
VL - 5
JO - Scientific Reports
JF - Scientific Reports
SN - 2045-2322
M1 - 10371
ER -