A methylation imprint mark in the mouse imprinted gene Grf1/Cdc25Mm locus shares a common feature with the U2afbp-rs gene: An association with a short tandem repeat and a hypermethylated region

Hideo Shibata, Yoshika Yoda, Reiko Kato, Takayuki Ueda, Mamoru Kamiya, Noriko Hiraiwa, Atsushi Yoshiki, Christoph Plass, R. Scott Pearsall, William A. Held, Masami Muramatsu, Hiroyuki Sasaki, Moriaki Kusakabe, Yoshihide Hayashizaki

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

We identified a sperm-specific methylation imprint mark (Site II) associated with a short tandem repeat sequence and a site/region methylated in both gametes (Site I) in the Grf1 locus on mouse chromosome 9, which shared a common feature with the U2afbp-rs gene. Sites or regions of gamete- specific methylation in imprinted genes are strong candidates for carrying information regarding the parental origin of alleles. The gamete-specific methylation pattern of Sites I and II was conserved after fertilization, but attained the somatic cell pattern by the blastocyst stage. In primordial germ cells, Site I was methylated, but Site II was unmethylated in both male and female embryos, suggesting that the sperm-specific methylation imprint mark in Site II was established during spermatogenesis. These common features in methylation imprint regions may be a clue to identifying regions carrying primary information for the imprinting regulation.

Original languageEnglish
Pages (from-to)30-37
Number of pages8
JournalGenomics
Volume49
Issue number1
DOIs
Publication statusPublished - Apr 1 1998

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ras-GRF1
Microsatellite Repeats
Methylation
Germ Cells
Genes
Spermatozoa
Chromosomes, Human, Pair 9
Tandem Repeat Sequences
Blastocyst
Spermatogenesis
Fertilization
Embryonic Structures
Alleles

All Science Journal Classification (ASJC) codes

  • Genetics

Cite this

A methylation imprint mark in the mouse imprinted gene Grf1/Cdc25Mm locus shares a common feature with the U2afbp-rs gene : An association with a short tandem repeat and a hypermethylated region. / Shibata, Hideo; Yoda, Yoshika; Kato, Reiko; Ueda, Takayuki; Kamiya, Mamoru; Hiraiwa, Noriko; Yoshiki, Atsushi; Plass, Christoph; Pearsall, R. Scott; Held, William A.; Muramatsu, Masami; Sasaki, Hiroyuki; Kusakabe, Moriaki; Hayashizaki, Yoshihide.

In: Genomics, Vol. 49, No. 1, 01.04.1998, p. 30-37.

Research output: Contribution to journalArticle

Shibata, H, Yoda, Y, Kato, R, Ueda, T, Kamiya, M, Hiraiwa, N, Yoshiki, A, Plass, C, Pearsall, RS, Held, WA, Muramatsu, M, Sasaki, H, Kusakabe, M & Hayashizaki, Y 1998, 'A methylation imprint mark in the mouse imprinted gene Grf1/Cdc25Mm locus shares a common feature with the U2afbp-rs gene: An association with a short tandem repeat and a hypermethylated region', Genomics, vol. 49, no. 1, pp. 30-37. https://doi.org/10.1006/geno.1998.5218
Shibata, Hideo ; Yoda, Yoshika ; Kato, Reiko ; Ueda, Takayuki ; Kamiya, Mamoru ; Hiraiwa, Noriko ; Yoshiki, Atsushi ; Plass, Christoph ; Pearsall, R. Scott ; Held, William A. ; Muramatsu, Masami ; Sasaki, Hiroyuki ; Kusakabe, Moriaki ; Hayashizaki, Yoshihide. / A methylation imprint mark in the mouse imprinted gene Grf1/Cdc25Mm locus shares a common feature with the U2afbp-rs gene : An association with a short tandem repeat and a hypermethylated region. In: Genomics. 1998 ; Vol. 49, No. 1. pp. 30-37.
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