A microfluidic molecular tracking system detects a time-to-search DNA sequence to be cleaved by restriction enzymes

D. Onoshima, N. Kaji, M. Tokeshi, Y. Baba

Research output: Contribution to conferencePaper

2 Citations (Scopus)

Abstract

A microfluidic analytical system, which uses simple technique to pick reacting DNA molecules out of a crowd of molecules during enzymatic reaction, was developed on the basis of single-molecule imaging. Restriction enzymes EcoR I and Not I with their target and non-target DNA were analyzed. With this system, we measured the duration difference of restriction-site-searching by these enzymes at the single-molecule level.

Original languageEnglish
Pages18-20
Number of pages3
Publication statusPublished - 2008
Externally publishedYes
Event12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008 - San Diego, CA, United States
Duration: Oct 12 2008Oct 16 2008

Other

Other12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008
CountryUnited States
CitySan Diego, CA
Period10/12/0810/16/08

All Science Journal Classification (ASJC) codes

  • Chemical Engineering (miscellaneous)
  • Bioengineering

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  • Cite this

    Onoshima, D., Kaji, N., Tokeshi, M., & Baba, Y. (2008). A microfluidic molecular tracking system detects a time-to-search DNA sequence to be cleaved by restriction enzymes. 18-20. Paper presented at 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008, San Diego, CA, United States.