A millisecond micro-RNA extraction technique for nanopore-based nucleic acid sequencing

Qiong Wu, Takao Yasui, Sakon Rahong, Takeshi Yanagida, Masaki Kanai, Noritada Kaji, Manabu Tokeshi, Kazuki Nagashima, Tomoji Kawai, Yoshinobu Baba

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

A ms(millisecond) miRNA(micro RNA) extraction from a mixture of total RNA and genomic DNA was successfully achieved by the combination of nanopillar and nanoslit structures inside a microchannel. This ultra-fast miRNA extraction technique especially useful for exosome-derived miRNA sequencing by nanopore-based RNA sequencer since exosomes contain miRNA and mRNA.

Original languageEnglish
Title of host publication18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
PublisherChemical and Biological Microsystems Society
Pages233-235
Number of pages3
ISBN (Electronic)9780979806476
Publication statusPublished - 2014
Externally publishedYes
Event18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 - San Antonio, United States
Duration: Oct 26 2014Oct 30 2014

Other

Other18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
CountryUnited States
CitySan Antonio
Period10/26/1410/30/14

All Science Journal Classification (ASJC) codes

  • Control and Systems Engineering

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  • Cite this

    Wu, Q., Yasui, T., Rahong, S., Yanagida, T., Kanai, M., Kaji, N., Tokeshi, M., Nagashima, K., Kawai, T., & Baba, Y. (2014). A millisecond micro-RNA extraction technique for nanopore-based nucleic acid sequencing. In 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 (pp. 233-235). Chemical and Biological Microsystems Society.