A millisecond micro-RNA separation technique by a hybrid structure of nanopillars and nanoslits

Qiong Wu, Noritada Kaji, Takao Yasui, Sakon Rahong, Takeshi Yanagida, Masaki Kanai, Kazuki Nagashima, Manabu Tokeshi, Tomoji Kawai, Yoshinobu Baba

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

A millisecond micro-RNA separation of a mixture of total RNA and genomic DNA, extracted from cultured HeLa cells, was successfully achieved using a hybrid structure of nanopillars and nanoslits contained inside a microchannel. The nanopillars, 250-nm in diameter and 100-nm in height, were fabricated with a 750-nm space inside the nanoslits, which were 100-nm in height and 25-μm in width; the nanopillars were then applied as a new sieve matrix. This ultra-fast technique for the separation of miRNA can be an effective pretreatment for semiconductor nanopore DNA sequencing, which has an optimum reading speed of 1 base/ms to obtain effective signal-to-noise ratio and discriminate each base by ion or tunneling current during the passage of nucleic acids.

Original languageEnglish
Article number43877
JournalScientific reports
Volume7
DOIs
Publication statusPublished - Mar 8 2017

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MicroRNAs
Nanopores
Semiconductors
Signal-To-Noise Ratio
DNA Sequence Analysis
HeLa Cells
Nucleic Acids
Reading
Cultured Cells
RNA
Ions
DNA

All Science Journal Classification (ASJC) codes

  • General

Cite this

A millisecond micro-RNA separation technique by a hybrid structure of nanopillars and nanoslits. / Wu, Qiong; Kaji, Noritada; Yasui, Takao; Rahong, Sakon; Yanagida, Takeshi; Kanai, Masaki; Nagashima, Kazuki; Tokeshi, Manabu; Kawai, Tomoji; Baba, Yoshinobu.

In: Scientific reports, Vol. 7, 43877, 08.03.2017.

Research output: Contribution to journalArticle

Wu, Qiong ; Kaji, Noritada ; Yasui, Takao ; Rahong, Sakon ; Yanagida, Takeshi ; Kanai, Masaki ; Nagashima, Kazuki ; Tokeshi, Manabu ; Kawai, Tomoji ; Baba, Yoshinobu. / A millisecond micro-RNA separation technique by a hybrid structure of nanopillars and nanoslits. In: Scientific reports. 2017 ; Vol. 7.
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AU - Kanai, Masaki

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