A mitochondrial delivery system using liposome-based nanocarriers that target myoblast cells

Takashi Katayama, Shintaro Kinugawa, Shingo Takada, Takaaki Furihata, A. Fukushima, Takashi Yokota, Toshihisa Anzai, Mitsue Hibino, Hideyoshi Harashima, Yuma Yamada

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Mitochondrial function is reduced in skeletal muscles of many patients with systemic diseases and it is difficult to deliver medicinal substances to mitochondria in such tissue. In this study, we report on attempts to develop liposome-based carriers for mitochondrial delivery using mouse myoblasts (C2C12) by varying the lipid composition of the carriers. We found that a liposome that contains an optimal lipid modified with the KALA peptide (a cellular uptake and mitochondrial targeting device) was the most effective nanocarrier for achieving mitochondrial delivery in C2C12 cells. We also report on successful mitochondrial transgene expression using the carriers encapsulating a mitochondrial DNA vector as we previously reported.

Original languageEnglish
Pages (from-to)66-72
Number of pages7
JournalMitochondrion
Volume49
DOIs
Publication statusPublished - Nov 2019

All Science Journal Classification (ASJC) codes

  • Molecular Medicine
  • Molecular Biology
  • Cell Biology

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    Katayama, T., Kinugawa, S., Takada, S., Furihata, T., Fukushima, A., Yokota, T., Anzai, T., Hibino, M., Harashima, H., & Yamada, Y. (2019). A mitochondrial delivery system using liposome-based nanocarriers that target myoblast cells. Mitochondrion, 49, 66-72. https://doi.org/10.1016/j.mito.2019.07.005