A Model of Transcriptional Activation of DNA by Loosening of Chromatin Structure

Yuko T. Sato; Daisuke Asai; Keiko Terada; Jeong Hun Kang; Takeshi Mori; Takuro Niidome; Hideki Nakashima; Yoshiki Katayama

doi:10.1246/cl.220392

A Model of Transcriptional Activation of DNA by Loosening of Chromatin Structure

Yuko T. Sato, Daisuke Asai, Keiko Terada, Jeong Hun Kang, Takeshi Mori, Takuro Niidome, Hideki Nakashima, Yoshiki Katayama

Chemistry and Biochemistry

Research output: Contribution to journal › Article › peer-review

Abstract

As a model of transcriptional activation of DNA by loosening of chromatin structure, we have developed a polymeric complex of plasmid DNA and a cationic polymer (SRC-Y) bearing peptide moieties that are phosphorylatable by p60c-Src. After phosphorylation, the diffusion constant of DAPIintercalated plasmid DNA in SRC-Y/DNA particles increased, concomitantly with initiation of transcription, meaning that the access of preinitiation complex, which recruits RNA polymerase II to promoter sequences, to chromatin-packaged DNA for transcriptional initiation is governed by DNA strand mobility.

Original language	English
Pages (from-to)	1109-1112
Number of pages	4
Journal	Chemistry Letters
Volume	51
Issue number	12
DOIs	https://doi.org/10.1246/cl.220392
Publication status	Published - Dec 2022

All Science Journal Classification (ASJC) codes

Chemistry(all)

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10.1246/cl.220392

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title = "A Model of Transcriptional Activation of DNA by Loosening of Chromatin Structure",

abstract = "As a model of transcriptional activation of DNA by loosening of chromatin structure, we have developed a polymeric complex of plasmid DNA and a cationic polymer (SRC-Y) bearing peptide moieties that are phosphorylatable by p60c-Src. After phosphorylation, the diffusion constant of DAPIintercalated plasmid DNA in SRC-Y/DNA particles increased, concomitantly with initiation of transcription, meaning that the access of preinitiation complex, which recruits RNA polymerase II to promoter sequences, to chromatin-packaged DNA for transcriptional initiation is governed by DNA strand mobility.",

author = "Sato, {Yuko T.} and Daisuke Asai and Keiko Terada and Kang, {Jeong Hun} and Takeshi Mori and Takuro Niidome and Hideki Nakashima and Yoshiki Katayama",

note = "Funding Information: We thank Mr. Kouki Miyamoto (Fujitsu Ltd) for his suggestions and technical assistance in the microinjection study, and Ms. Shigemi Terakubo and Ms. Satomi Yamazaki (St. Marianna University School of Medicine) for their technical assistance. This work was supported by open innovation platform for industry-academia co-creation. Publisher Copyright: {\textcopyright} 2022 The Chemical Society of Japan.",

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AU - Sato, Yuko T.

AU - Asai, Daisuke

AU - Terada, Keiko

AU - Kang, Jeong Hun

AU - Mori, Takeshi

AU - Niidome, Takuro

AU - Nakashima, Hideki

AU - Katayama, Yoshiki

N1 - Funding Information: We thank Mr. Kouki Miyamoto (Fujitsu Ltd) for his suggestions and technical assistance in the microinjection study, and Ms. Shigemi Terakubo and Ms. Satomi Yamazaki (St. Marianna University School of Medicine) for their technical assistance. This work was supported by open innovation platform for industry-academia co-creation. Publisher Copyright: © 2022 The Chemical Society of Japan.

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N2 - As a model of transcriptional activation of DNA by loosening of chromatin structure, we have developed a polymeric complex of plasmid DNA and a cationic polymer (SRC-Y) bearing peptide moieties that are phosphorylatable by p60c-Src. After phosphorylation, the diffusion constant of DAPIintercalated plasmid DNA in SRC-Y/DNA particles increased, concomitantly with initiation of transcription, meaning that the access of preinitiation complex, which recruits RNA polymerase II to promoter sequences, to chromatin-packaged DNA for transcriptional initiation is governed by DNA strand mobility.

AB - As a model of transcriptional activation of DNA by loosening of chromatin structure, we have developed a polymeric complex of plasmid DNA and a cationic polymer (SRC-Y) bearing peptide moieties that are phosphorylatable by p60c-Src. After phosphorylation, the diffusion constant of DAPIintercalated plasmid DNA in SRC-Y/DNA particles increased, concomitantly with initiation of transcription, meaning that the access of preinitiation complex, which recruits RNA polymerase II to promoter sequences, to chromatin-packaged DNA for transcriptional initiation is governed by DNA strand mobility.

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A Model of Transcriptional Activation of DNA by Loosening of Chromatin Structure

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