A molecular mechanism for autoinhibition of the tandem SH3 domains of p47phox, the regulatory subunit of the phagocyte NADPH oxidase

Satoru Yuzawa, Nobuo N. Suzuki, Yuko Fujioka, Kenji Ogura, Hideki Sumimoto, Fuyuhiko Inagaki

Research output: Contribution to journalArticle

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Abstract

The phagocyte NADPH oxidase is a multisubunit enzyme responsible for the production of reactive oxygen species. p47phox is a cytosolic component of the NADPH oxidase and plays an important role in the assembly of the activated complex. The structural determination of the tandem SH3 domains of p47phox is crucial for elucidation of the molecular mechanism of the activation of P47phox. We determined the X-ray crystal structure of the tandem SH3 domains with the polybasic/autoinhibitory region (PBR/AIR) of p47phox. The GAPPR sequence involved in PBR/AIR forms a left-handed polyproline type-II helix (PPII) and interacts with the conserved SH3 binding surfaces of the SH3 domains simultaneously. These SH3 domains are related by a 2-fold pseudosymmetry axis at the centre of the binding groove and interact with the single PPII helix formed by the GAPPR sequence with opposite orientation. In addition, a number of intra-molecular interactions among the SH3 domains, PBR/AIR and the linker tightly hold the architecture of the tandem SH3 domains into the compact structure and stabilize the autoinhibited form synergistically. Phosphorylation of the serine residues in PBR/AIR could destabilize and successively release the intra-molecular interactions. Thus, the overall structure could be rearranged from the autoinhibitory conformation to the active conformation and the PPII ligand binding surfaces on the SH3 domains are now unmasked, which enables their interaction with the target sequence in p22phox.

Original languageEnglish
Pages (from-to)443-456
Number of pages14
JournalGenes to Cells
Volume9
Issue number5
DOIs
Publication statusPublished - May 1 2004

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src Homology Domains
NADPH Oxidase
Phagocytes
Serine
Reactive Oxygen Species
Phosphorylation
X-Rays
Ligands
Enzymes

All Science Journal Classification (ASJC) codes

  • Genetics
  • Cell Biology

Cite this

A molecular mechanism for autoinhibition of the tandem SH3 domains of p47phox, the regulatory subunit of the phagocyte NADPH oxidase. / Yuzawa, Satoru; Suzuki, Nobuo N.; Fujioka, Yuko; Ogura, Kenji; Sumimoto, Hideki; Inagaki, Fuyuhiko.

In: Genes to Cells, Vol. 9, No. 5, 01.05.2004, p. 443-456.

Research output: Contribution to journalArticle

Yuzawa, Satoru ; Suzuki, Nobuo N. ; Fujioka, Yuko ; Ogura, Kenji ; Sumimoto, Hideki ; Inagaki, Fuyuhiko. / A molecular mechanism for autoinhibition of the tandem SH3 domains of p47phox, the regulatory subunit of the phagocyte NADPH oxidase. In: Genes to Cells. 2004 ; Vol. 9, No. 5. pp. 443-456.
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