A molecularly imprinted polymer that shows enzymatic activity

Eiichi Toorisaka, Kazuya Uezu, Masahiro Goto, Shintaro Furusaki

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

An enzyme-mimic polymer was prepared by the surface molecular imprinting technique. In the active site of the polymer, a substrate analog was imprinted through the complex formation between a cobalt ion and alkyl imidazole that functions as the hydrolysis catalysis. The enzymatic performance of the imprinted polymer was evaluated by the hydrolysis reaction of an amino acid ester. Based on the Michaelis-Menten analysis, Vmax and Km were obtained. The imprinted polymer shows a high activity compared to that of the control unimprinted polymer. The result was supported by a low Km value of the imprinted polymer, indicating a high affinity to the target substrate. The enzyme-mimic polymer was found to possess a substrate specificity by using several substrates that have a different structure. A computational modeling also supports the structure of the imprinted sites formed on the polymer surface.

Original languageEnglish
Pages (from-to)85-91
Number of pages7
JournalBiochemical Engineering Journal
Volume14
Issue number2
DOIs
Publication statusPublished - May 1 2003

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Polymers
Substrates
Hydrolysis
Enzymes
Molecular Imprinting
Substrate Specificity
Cobalt
Catalysis
Amino acids
Catalytic Domain
Esters
Ions
Amino Acids

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Environmental Engineering
  • Bioengineering
  • Biomedical Engineering

Cite this

A molecularly imprinted polymer that shows enzymatic activity. / Toorisaka, Eiichi; Uezu, Kazuya; Goto, Masahiro; Furusaki, Shintaro.

In: Biochemical Engineering Journal, Vol. 14, No. 2, 01.05.2003, p. 85-91.

Research output: Contribution to journalArticle

Toorisaka, Eiichi ; Uezu, Kazuya ; Goto, Masahiro ; Furusaki, Shintaro. / A molecularly imprinted polymer that shows enzymatic activity. In: Biochemical Engineering Journal. 2003 ; Vol. 14, No. 2. pp. 85-91.
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