A mouse B16 melanoma mutant deficient in glycolipids

Shinichi Ichikawa, Nobushige Nakajo, Hisako Sakiyama, Yoshio Hirabayashi

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139 Citations (Scopus)

Abstract

Mouse B16 melanoma cell line, GM-95 (formerly designated as MEC-4), deficient in sialyllactosylceramide was examined for its primary defect. Glycolipids from the mutant cells were analyzed by high-performance TLC. No glycolipid was detected in GM-95 cells, even when total lipid from 107 cells was analyzed. In contrast, the content of ceramide, a precursor lipid molecule of glycolipids, was normal. Thus, the deficiency of glycolipids was attributed to the first glucosylation step of ceramide. The ceramide glucosyltransferase (EC 2.4.1.80) activity was not detected in GM-95 cells. There was no significant difference of sialyllactosylceramide synthase activity, however, between GM-95 and the parental cells. The deficiency of glycolipids in GM-95 cells was associated with changes of the cellular morphology and growth rate. The parental cells showed irregular shapes and tended to overlap each other. On the other hand, GM-95 cells exhibited an elongated fibroblastic morphology and parallel arrangement. The population- doubling times of GM-95 and the parental cells in serum-free medium were 28 hr and 19 hr, respectively.

Original languageEnglish
Pages (from-to)2703-2707
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume91
Issue number7
DOIs
Publication statusPublished - Mar 29 1994

All Science Journal Classification (ASJC) codes

  • General

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