A multifunctional flow-injection biosensor for the simultaneous determination of ammonia, creatinine, and urea

C. S. Rui, H. I-Ogawa, Kenji Sonomoto, Y. Kato

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

A strategy for the multifunctionalization of the FIA biosensor was developed. The described multifunctional FIA system offers a fast and simple method for the simultaneous determination of ammonia, creatinine, and urea. The hydrolysis of creatinine by creatinine deiminase (CRDI) or of urea by urease forms ammonia, which is amperometrically detected by an oxygen electrode, based on an enzyme conversion system, glutamate dehydrogenase (GLDH)/glutamate oxidase (GLOD). The split of the stream into three after sample injection and confluence before the GLDH reactor resulted in a three-channel system, into which were set three parallel columns, respectively, filled with immobilized CRDI, urease, and CPG. A triple-peak recording was obtained by putting two delay coils at the channels involving CRDI and urease. Thus the interfering of the endogenous ammonia on the creatinine and urea assay is simultaneously compensated. Furthermore, the problem of great difference in concentration between urea and the other two components is resolved by taking advantage of the differentiated dilution effect for each channel caused from the split-stream, flow-injection system. Linear calibration ranges for ammonia, creatinine, and urea were 0.1-5, 0.2-10, and 2-40 mM, respectively. One run was finished within 5 minutes, and the system was reproducibility good (3 to 5%). The results of the urine assay obtained by the present method will be described in the near future.

Original languageEnglish
Pages (from-to)30-38
Number of pages9
JournalAnnals of the New York Academy of Sciences
Volume750
DOIs
Publication statusPublished - Jan 1 1995
Externally publishedYes

Fingerprint

creatinine deiminase
Biosensing Techniques
Ammonia
Biosensors
Urea
Creatinine
Urease
Injections
Glutamate Dehydrogenase
Assays
Stream flow
Calibration
Dilution
Glutamic Acid
Hydrolysis
Oxidoreductases
Electrodes
Urine
Oxygen
Enzymes

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • History and Philosophy of Science

Cite this

A multifunctional flow-injection biosensor for the simultaneous determination of ammonia, creatinine, and urea. / Rui, C. S.; I-Ogawa, H.; Sonomoto, Kenji; Kato, Y.

In: Annals of the New York Academy of Sciences, Vol. 750, 01.01.1995, p. 30-38.

Research output: Contribution to journalArticle

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