A Munc13-like protein in Arabidopsis mediates H+-ATPase translocation that is essential for stomatal responses

Mimi Hashimoto-Sugimoto, Takumi Higaki, Takashi Yaeno, Ayako Nagami, Mari Irie, Miho Fujimi, Megumi Miyamoto, Kae Akita, Juntaro Negi, Ken Shirasu, Seiichiro Hasezawa, Koh Iba

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

Plants control CO2 uptake and water loss by modulating the aperture of stomata located in the epidermis. Stomatal opening is initiated by the activation of H +-ATPases in the guard-cell plasma membrane. In contrast to regulation of H +-ATPase activity, little is known about the translocation of the guard cell H +-ATPase to the plasma membrane. Here we describe the isolation of an Arabidopsis gene, PATROL1, that controls the translocation of a major H +-ATPase, AHA1, to the plasma membrane. PATROL1 encodes a protein with a MUN domain, known to mediate synaptic priming in neuronal exocytosis in animals. Environmental stimuli change the localization of plasma membrane-associated PATROL1 to an intracellular compartment. Plasma membrane localization of AHA1 and stomatal opening require the association of PATROL1 with AHA1. Increased stomatal opening responses in plants overexpressing PATROL1 enhance the CO 2 assimilation rate, promoting plant growth.

Original languageEnglish
Article number3215
JournalNature communications
Volume4
DOIs
Publication statusPublished - Aug 15 2013

Fingerprint

Arabidopsis Proteins
Proton-Translocating ATPases
Cell membranes
Cell Membrane
membranes
proteins
Proteins
priming
water loss
epidermis
assimilation
compartments
cells
genes
stimuli
Exocytosis
animals
Carbon Monoxide
isolation
Arabidopsis

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

Cite this

A Munc13-like protein in Arabidopsis mediates H+-ATPase translocation that is essential for stomatal responses. / Hashimoto-Sugimoto, Mimi; Higaki, Takumi; Yaeno, Takashi; Nagami, Ayako; Irie, Mari; Fujimi, Miho; Miyamoto, Megumi; Akita, Kae; Negi, Juntaro; Shirasu, Ken; Hasezawa, Seiichiro; Iba, Koh.

In: Nature communications, Vol. 4, 3215, 15.08.2013.

Research output: Contribution to journalArticle

Hashimoto-Sugimoto, M, Higaki, T, Yaeno, T, Nagami, A, Irie, M, Fujimi, M, Miyamoto, M, Akita, K, Negi, J, Shirasu, K, Hasezawa, S & Iba, K 2013, 'A Munc13-like protein in Arabidopsis mediates H+-ATPase translocation that is essential for stomatal responses', Nature communications, vol. 4, 3215. https://doi.org/10.1038/ncomms3215
Hashimoto-Sugimoto, Mimi ; Higaki, Takumi ; Yaeno, Takashi ; Nagami, Ayako ; Irie, Mari ; Fujimi, Miho ; Miyamoto, Megumi ; Akita, Kae ; Negi, Juntaro ; Shirasu, Ken ; Hasezawa, Seiichiro ; Iba, Koh. / A Munc13-like protein in Arabidopsis mediates H+-ATPase translocation that is essential for stomatal responses. In: Nature communications. 2013 ; Vol. 4.
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