A mutant rat strain defficient in induction of a phenobarbital-inducible form of cytochrome P-450 in liver microsomes

Toshihide Hashimoto, Tatsumi Matsumoto, Mayumi Nishizawa, Shun Ichiro Kawabata, Ken Ichirou Morohashi, Sumio Handa, Tsuneo Omura

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18 Citations (Scopus)

Abstract

Two phenobarbital-inducible forms of cytochrome P-450, P-450(PB-1), and P-450(PB-4), were purified from the liver microsomes of phenobarbital-treated rats and identified with P-450b and P-450e, respectively. It was found, however, that the content of P-450(PB-4) in the liver microsomes of a strain of SD rat, Qdj: SD, was very low even after phenobarbital-induction. The levels of the mRNAs for P-450(PB-1) and P-450(PB-4) were separately determined using Northern blot hybridization with specific oligonucleotide probes. It was found that the level of P-450(PB-4) mRNA in the livers of phenobarbital-treated Qdj: SD rats was much lower than that of phenobarbital-treated Slc: SD rats. Slc:SD rats are widely used in Japanese laboratories. Genetic analysis using the crossbred animals between Qdj: SD and Slc: SD rats showed that the low expression of P-450(PB-4) in Qdj: SD rats is a recessive trait and is caused by a single gene mutation. However, no difference in the 5' flanking region in P-450(PB-4) gene was found between Qdj: SD rats and Slc: SD rats.

Original languageEnglish
Pages (from-to)487-492
Number of pages6
JournalJournal of biochemistry
Volume103
Issue number3
DOIs
Publication statusPublished - Mar 1988

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

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