A MutS protein-immobilized Au electrode for detecting single-base mismatch of DNA

Aishan Han, Tohru Takarada, Taiki Shibata, Masamichi Nakayama, Mizuo Maeda

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

A novel electrochemical biosensor was developed to detect gene mutation by using a DNA-mismatch binding protein: MutS from Escherichia coli. The MutS protein was immobilized onto an Au-electrode surface via complex formation between a histidine tag of the MutS protein and a thiol-modified nitrilotriacetic acid chemically adsorbed on the Au-electrode surface. When a target double-stranded DNA having a single-base mismatch was captured by the MutS protein on the electrode, some electrostatic repulsion arose between polyanionic DNA strands and anionic redox couple ions. Consequently, their redox peak currents on a cyclic voltammogram with the Au electrode drastically decreased, depending on the concentration of the target DNA, according to the redox couple-mediated artificial ion-channel principle. By using this assay, one can detect all types of single-base mismatch and single-base deletion. 2006

Original languageEnglish
Pages (from-to)663-666
Number of pages4
Journalanalytical sciences
Volume22
Issue number5
DOIs
Publication statusPublished - May 2006
Externally publishedYes

Fingerprint

Immobilized Proteins
Electrodes
DNA
MutS DNA Mismatch-Binding Protein
Nitrilotriacetic Acid
Proteins
Ion Channels
Histidine
Sulfhydryl Compounds
Biosensors
Escherichia coli
Electrostatics
Assays
Genes
Ions
Oxidation-Reduction

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry

Cite this

A MutS protein-immobilized Au electrode for detecting single-base mismatch of DNA. / Han, Aishan; Takarada, Tohru; Shibata, Taiki; Nakayama, Masamichi; Maeda, Mizuo.

In: analytical sciences, Vol. 22, No. 5, 05.2006, p. 663-666.

Research output: Contribution to journalArticle

Han, Aishan ; Takarada, Tohru ; Shibata, Taiki ; Nakayama, Masamichi ; Maeda, Mizuo. / A MutS protein-immobilized Au electrode for detecting single-base mismatch of DNA. In: analytical sciences. 2006 ; Vol. 22, No. 5. pp. 663-666.
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