A nano-sized gel-in-oil suspension for transcutaneous protein delivery

Safrina Dyah Hardiningtyas, Seiya Nagao, Emiko Yamamoto, Nana Shirakigawa, Rie Wakabayashi, Masahiro Goto, Hiroyuki Ijima, Noriho Kamiya

Research output: Contribution to journalArticle

Abstract

We developed a new oil-based delivery system for transdermal protein delivery, a gel-in-oil (G/O) nanosuspension, where gelatin-based hydrogel was coated with hydrophobic surfactants. The high entrapment efficiency of a model protein, phycocyanin (PC), into nano-sized gelatin hydrogel particles was achieved. Spectroscopic evaluation of PC suggested that the G/O nanosuspension could retain the functional form of PC in isopropyl myristate. In vitro skin permeation studies showed that the G/O nanosuspension facilitated the delivery of PC through the stratum corneum of Yucatan micropig skin.

Original languageEnglish
Article number118495
JournalInternational Journal of Pharmaceutics
Volume567
DOIs
Publication statusPublished - Aug 15 2019

Fingerprint

Phycocyanin
Suspensions
Oils
Gels
Hydrogel
Gelatin
Proteins
Skin
Surface-Active Agents
Cornea

All Science Journal Classification (ASJC) codes

  • Pharmaceutical Science

Cite this

A nano-sized gel-in-oil suspension for transcutaneous protein delivery. / Hardiningtyas, Safrina Dyah; Nagao, Seiya; Yamamoto, Emiko; Shirakigawa, Nana; Wakabayashi, Rie; Goto, Masahiro; Ijima, Hiroyuki; Kamiya, Noriho.

In: International Journal of Pharmaceutics, Vol. 567, 118495, 15.08.2019.

Research output: Contribution to journalArticle

@article{f8ff367ce8134c6c91425c3c9350e1d0,
title = "A nano-sized gel-in-oil suspension for transcutaneous protein delivery",
abstract = "We developed a new oil-based delivery system for transdermal protein delivery, a gel-in-oil (G/O) nanosuspension, where gelatin-based hydrogel was coated with hydrophobic surfactants. The high entrapment efficiency of a model protein, phycocyanin (PC), into nano-sized gelatin hydrogel particles was achieved. Spectroscopic evaluation of PC suggested that the G/O nanosuspension could retain the functional form of PC in isopropyl myristate. In vitro skin permeation studies showed that the G/O nanosuspension facilitated the delivery of PC through the stratum corneum of Yucatan micropig skin.",
author = "Hardiningtyas, {Safrina Dyah} and Seiya Nagao and Emiko Yamamoto and Nana Shirakigawa and Rie Wakabayashi and Masahiro Goto and Hiroyuki Ijima and Noriho Kamiya",
year = "2019",
month = "8",
day = "15",
doi = "10.1016/j.ijpharm.2019.118495",
language = "English",
volume = "567",
journal = "International Journal of Pharmaceutics",
issn = "0378-5173",
publisher = "Elsevier",

}

TY - JOUR

T1 - A nano-sized gel-in-oil suspension for transcutaneous protein delivery

AU - Hardiningtyas, Safrina Dyah

AU - Nagao, Seiya

AU - Yamamoto, Emiko

AU - Shirakigawa, Nana

AU - Wakabayashi, Rie

AU - Goto, Masahiro

AU - Ijima, Hiroyuki

AU - Kamiya, Noriho

PY - 2019/8/15

Y1 - 2019/8/15

N2 - We developed a new oil-based delivery system for transdermal protein delivery, a gel-in-oil (G/O) nanosuspension, where gelatin-based hydrogel was coated with hydrophobic surfactants. The high entrapment efficiency of a model protein, phycocyanin (PC), into nano-sized gelatin hydrogel particles was achieved. Spectroscopic evaluation of PC suggested that the G/O nanosuspension could retain the functional form of PC in isopropyl myristate. In vitro skin permeation studies showed that the G/O nanosuspension facilitated the delivery of PC through the stratum corneum of Yucatan micropig skin.

AB - We developed a new oil-based delivery system for transdermal protein delivery, a gel-in-oil (G/O) nanosuspension, where gelatin-based hydrogel was coated with hydrophobic surfactants. The high entrapment efficiency of a model protein, phycocyanin (PC), into nano-sized gelatin hydrogel particles was achieved. Spectroscopic evaluation of PC suggested that the G/O nanosuspension could retain the functional form of PC in isopropyl myristate. In vitro skin permeation studies showed that the G/O nanosuspension facilitated the delivery of PC through the stratum corneum of Yucatan micropig skin.

UR - http://www.scopus.com/inward/record.url?scp=85068534746&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85068534746&partnerID=8YFLogxK

U2 - 10.1016/j.ijpharm.2019.118495

DO - 10.1016/j.ijpharm.2019.118495

M3 - Article

AN - SCOPUS:85068534746

VL - 567

JO - International Journal of Pharmaceutics

JF - International Journal of Pharmaceutics

SN - 0378-5173

M1 - 118495

ER -