A new blood-compatible surface prepared by poly(2-methoxyethylacrylate) (PMEA) coating - Protein adsorption on PMEA surface

Masaru Tanaka, T. Motomura, M. Kawada, T. Anzai, Y. Kasori, K. Shimura, M. Onishi, A. Mochizuki, Y. Okahata

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Poly(2-methoxyethylacrylate) (PMEA) surface shows good blood compatibility with respect to the coagulation, complement, and platelet systems when compared with other polymer surfaces. To clarify the reasons for this good compatibility, the amount of the plasma protein adsorbed onto PMEA and its secondary structure were investigated. Poly (2-hydroxyethylmethacrylate) (PHEMA) and polyacrylate analogs were used as references. The amount of protein adsorbed onto PMEA was very small, and close to that adsorbed onto PHEMA. Circular dichroism (CD) spectroscopy revealed that the conformations of the proteins adsorbed onto PHEMA changed considerably, while those of the proteins adsorbed onto PMEA differed only slightly from the native one. Based on the quartz crystal microbalance (QCM) measurement, we estimated the binding constant and association and dissociation rate constants of the proteins adsorbed onto the polymer surfaces. These results suggested that the excellent blood compatibility of PMEA is closely related to the low denaturation and the high dissociation rate constant of the proteins adsorbed onto PMEA. In addition, the structure of water in the hydrated PMEA was investigated using differential scanning calorimetry (DSC). Cold crystallization of water in the heating process was clearly observed at -42°C. This cold crystallization is interpreted as the phase transition from the amorphous ice to the crystal ice that belongs to the freeze-bound water in PMEA. We believe that this feature of the water structure is related to blood compatibility.

Original languageEnglish
Pages (from-to)209-216
Number of pages8
JournalJapanese Journal of Artificial Organs
Volume29
Issue number1
Publication statusPublished - Dec 1 2000
Externally publishedYes

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Adsorption
Water
Ice
Crystallization
Polymers
Proteins
Quartz Crystal Microbalance Techniques
Protein Conformation
Phase Transition
Differential Scanning Calorimetry
Circular Dichroism
Heating
Blood Proteins
Spectrum Analysis
Blood Platelets
poly(2-methoxyethylacrylate)

All Science Journal Classification (ASJC) codes

  • Biophysics

Cite this

A new blood-compatible surface prepared by poly(2-methoxyethylacrylate) (PMEA) coating - Protein adsorption on PMEA surface. / Tanaka, Masaru; Motomura, T.; Kawada, M.; Anzai, T.; Kasori, Y.; Shimura, K.; Onishi, M.; Mochizuki, A.; Okahata, Y.

In: Japanese Journal of Artificial Organs, Vol. 29, No. 1, 01.12.2000, p. 209-216.

Research output: Contribution to journalArticle

Tanaka, M, Motomura, T, Kawada, M, Anzai, T, Kasori, Y, Shimura, K, Onishi, M, Mochizuki, A & Okahata, Y 2000, 'A new blood-compatible surface prepared by poly(2-methoxyethylacrylate) (PMEA) coating - Protein adsorption on PMEA surface', Japanese Journal of Artificial Organs, vol. 29, no. 1, pp. 209-216.
Tanaka, Masaru ; Motomura, T. ; Kawada, M. ; Anzai, T. ; Kasori, Y. ; Shimura, K. ; Onishi, M. ; Mochizuki, A. ; Okahata, Y. / A new blood-compatible surface prepared by poly(2-methoxyethylacrylate) (PMEA) coating - Protein adsorption on PMEA surface. In: Japanese Journal of Artificial Organs. 2000 ; Vol. 29, No. 1. pp. 209-216.
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