A new cancer cell detection method using an infectivity-enhanced adenoviral vector

Junji Uchino, Koichi Takayama, Noriaki Nakagaki, Wang Shuo, Junko Hisasue, Keita Nakatom, Keiichi Ohta, Ryosuke Hirano, Naoki Tashiro, Izumi Miiru, Masaki Fujita, Kentaro Watanabe, Yoichi Nakanishi

Research output: Contribution to journalArticle

Abstract

Cytological examination is widely used as a diagnostic tool because of the ease of collecting cells from the involved area. However, the diagnostic yield of cytological examination is unsatisfactory; the reasons include sampling error, poorly prepared samples, small numbers of malignant cells, and low grades of cellular atypia. In this study, we focused on the high infectivity of adenovirus towards epithelial cells and applied the luciferase-expressing adenoviral vector to a new cancer cell detection tool. In addition, adenoviral infectivity was enhanced by modifying viral fiber proteins. The sensitivity of the diagnostic tool was tested using the NCI-H1299 lung cancer cell line, and validated in body fluid samples from cancer patients with a variety of etiology. Results showed that the adenovirus efficiently transfected NCI-H1299 with high sensitivity. Only 10 cancer cells were sufficient for detection of luciferase signals. In body fluid samples, the adenovirus confirmed the diagnosis for malignant and benign cancer, but not in non-epithelial cell derived samples. This study provides proof-of-concept for a more reliable and sensitive diagnostic tool for epithelium-derived cancer.

Original languageEnglish
Pages (from-to)5551-5556
Number of pages6
JournalAsian Pacific Journal of Cancer Prevention
Volume13
Issue number11
DOIs
Publication statusPublished - 2012

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All Science Journal Classification (ASJC) codes

  • Epidemiology
  • Oncology
  • Public Health, Environmental and Occupational Health
  • Cancer Research

Cite this

Uchino, J., Takayama, K., Nakagaki, N., Shuo, W., Hisasue, J., Nakatom, K., ... Nakanishi, Y. (2012). A new cancer cell detection method using an infectivity-enhanced adenoviral vector. Asian Pacific Journal of Cancer Prevention, 13(11), 5551-5556. https://doi.org/10.7314/APJCP.2012.13.11.5551