A new ETV6/TEL partner gene, ARG (ABL-related gene or ABL2), identified in an AML-M3 cell line with a t(1;12)(q25;p13) translocation

Yoshimi Iijima, Takashi Ito, Tsuneyuki Oikawa, Mariko Eguchi, Minenori Eguchi-Ishimae, Nanao Kamada, Kenji Kishi, Shigetaka Asano, Yoshiyuki Sakaki, Yuko Sato

Research output: Contribution to journalArticle

85 Citations (Scopus)

Abstract

The ETV6/TEL gene has been reported to fuse to PDGFRβb MDS1/EV11, BTL, ACS2, STL, JAK2, ABL, CDX2, TRKC, AML1, and MN1. Among them, PDGFRβ, ABL, JAK2, and TRKC are tyrosine kinases (TK). We identified a novel ETV6 partner gene, ARG (ABL-related gene or ABL2), another TK gene in a cell line established from a patient with acute myelogenous leukemia (AML-M3) with a t(15;17)(q22;q11.2) and a t(1;12)(q25;p13), which has the remarkable feature to differentiate to mature eosinophils in culture with all-trans retinoic acid and cytokines. The ETV6/ARG transcripts consisted of exon 1 to 5 of ETV6 and the 3' portion of ARG starting from exon 1B or exon 2, resulting in an open reading frame for a fusion protein consisting of the entire PNT oligomerization domain of ETV6 and all of the functional domains of ARG including the TK domain. This is the same protein structure as identified in the other ETV6 TK fusion proteins. The reciprocal ARG! ETV6 transcript was not expressed, and the normal ETV6 allele was not deleted or rearranged. Although the ABL is known to be involved in various human malignancies, ARG has not been involved in human malignancies despite its high homology to ABL. Thus, this is the first report showing involvement of ARG in human leukemia. The ETV6/ARG protein may be involved in the unique differentiation capacity of this cell line. (C) 2000 by The American Society of Hematology.

Original languageEnglish
Pages (from-to)2126-2131
Number of pages6
JournalBlood
Volume95
Issue number6
Publication statusPublished - Mar 15 2000
Externally publishedYes

Fingerprint

Acute Promyelocytic Leukemia
Protein-Tyrosine Kinases
Genes
Cells
Cell Line
Exons
Proteins
Fusion reactions
Oligomerization
Electric fuses
Tretinoin
Eosinophils
Acute Myeloid Leukemia
Open Reading Frames
Neoplasms
Leukemia
Alleles
Cytokines

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Immunology
  • Hematology
  • Cell Biology

Cite this

Iijima, Y., Ito, T., Oikawa, T., Eguchi, M., Eguchi-Ishimae, M., Kamada, N., ... Sato, Y. (2000). A new ETV6/TEL partner gene, ARG (ABL-related gene or ABL2), identified in an AML-M3 cell line with a t(1;12)(q25;p13) translocation. Blood, 95(6), 2126-2131.

A new ETV6/TEL partner gene, ARG (ABL-related gene or ABL2), identified in an AML-M3 cell line with a t(1;12)(q25;p13) translocation. / Iijima, Yoshimi; Ito, Takashi; Oikawa, Tsuneyuki; Eguchi, Mariko; Eguchi-Ishimae, Minenori; Kamada, Nanao; Kishi, Kenji; Asano, Shigetaka; Sakaki, Yoshiyuki; Sato, Yuko.

In: Blood, Vol. 95, No. 6, 15.03.2000, p. 2126-2131.

Research output: Contribution to journalArticle

Iijima, Y, Ito, T, Oikawa, T, Eguchi, M, Eguchi-Ishimae, M, Kamada, N, Kishi, K, Asano, S, Sakaki, Y & Sato, Y 2000, 'A new ETV6/TEL partner gene, ARG (ABL-related gene or ABL2), identified in an AML-M3 cell line with a t(1;12)(q25;p13) translocation', Blood, vol. 95, no. 6, pp. 2126-2131.
Iijima Y, Ito T, Oikawa T, Eguchi M, Eguchi-Ishimae M, Kamada N et al. A new ETV6/TEL partner gene, ARG (ABL-related gene or ABL2), identified in an AML-M3 cell line with a t(1;12)(q25;p13) translocation. Blood. 2000 Mar 15;95(6):2126-2131.
Iijima, Yoshimi ; Ito, Takashi ; Oikawa, Tsuneyuki ; Eguchi, Mariko ; Eguchi-Ishimae, Minenori ; Kamada, Nanao ; Kishi, Kenji ; Asano, Shigetaka ; Sakaki, Yoshiyuki ; Sato, Yuko. / A new ETV6/TEL partner gene, ARG (ABL-related gene or ABL2), identified in an AML-M3 cell line with a t(1;12)(q25;p13) translocation. In: Blood. 2000 ; Vol. 95, No. 6. pp. 2126-2131.
@article{e41ef19ae1404be48896a130fd4f2461,
title = "A new ETV6/TEL partner gene, ARG (ABL-related gene or ABL2), identified in an AML-M3 cell line with a t(1;12)(q25;p13) translocation",
abstract = "The ETV6/TEL gene has been reported to fuse to PDGFRβb MDS1/EV11, BTL, ACS2, STL, JAK2, ABL, CDX2, TRKC, AML1, and MN1. Among them, PDGFRβ, ABL, JAK2, and TRKC are tyrosine kinases (TK). We identified a novel ETV6 partner gene, ARG (ABL-related gene or ABL2), another TK gene in a cell line established from a patient with acute myelogenous leukemia (AML-M3) with a t(15;17)(q22;q11.2) and a t(1;12)(q25;p13), which has the remarkable feature to differentiate to mature eosinophils in culture with all-trans retinoic acid and cytokines. The ETV6/ARG transcripts consisted of exon 1 to 5 of ETV6 and the 3' portion of ARG starting from exon 1B or exon 2, resulting in an open reading frame for a fusion protein consisting of the entire PNT oligomerization domain of ETV6 and all of the functional domains of ARG including the TK domain. This is the same protein structure as identified in the other ETV6 TK fusion proteins. The reciprocal ARG! ETV6 transcript was not expressed, and the normal ETV6 allele was not deleted or rearranged. Although the ABL is known to be involved in various human malignancies, ARG has not been involved in human malignancies despite its high homology to ABL. Thus, this is the first report showing involvement of ARG in human leukemia. The ETV6/ARG protein may be involved in the unique differentiation capacity of this cell line. (C) 2000 by The American Society of Hematology.",
author = "Yoshimi Iijima and Takashi Ito and Tsuneyuki Oikawa and Mariko Eguchi and Minenori Eguchi-Ishimae and Nanao Kamada and Kenji Kishi and Shigetaka Asano and Yoshiyuki Sakaki and Yuko Sato",
year = "2000",
month = "3",
day = "15",
language = "English",
volume = "95",
pages = "2126--2131",
journal = "Blood",
issn = "0006-4971",
publisher = "American Society of Hematology",
number = "6",

}

TY - JOUR

T1 - A new ETV6/TEL partner gene, ARG (ABL-related gene or ABL2), identified in an AML-M3 cell line with a t(1;12)(q25;p13) translocation

AU - Iijima, Yoshimi

AU - Ito, Takashi

AU - Oikawa, Tsuneyuki

AU - Eguchi, Mariko

AU - Eguchi-Ishimae, Minenori

AU - Kamada, Nanao

AU - Kishi, Kenji

AU - Asano, Shigetaka

AU - Sakaki, Yoshiyuki

AU - Sato, Yuko

PY - 2000/3/15

Y1 - 2000/3/15

N2 - The ETV6/TEL gene has been reported to fuse to PDGFRβb MDS1/EV11, BTL, ACS2, STL, JAK2, ABL, CDX2, TRKC, AML1, and MN1. Among them, PDGFRβ, ABL, JAK2, and TRKC are tyrosine kinases (TK). We identified a novel ETV6 partner gene, ARG (ABL-related gene or ABL2), another TK gene in a cell line established from a patient with acute myelogenous leukemia (AML-M3) with a t(15;17)(q22;q11.2) and a t(1;12)(q25;p13), which has the remarkable feature to differentiate to mature eosinophils in culture with all-trans retinoic acid and cytokines. The ETV6/ARG transcripts consisted of exon 1 to 5 of ETV6 and the 3' portion of ARG starting from exon 1B or exon 2, resulting in an open reading frame for a fusion protein consisting of the entire PNT oligomerization domain of ETV6 and all of the functional domains of ARG including the TK domain. This is the same protein structure as identified in the other ETV6 TK fusion proteins. The reciprocal ARG! ETV6 transcript was not expressed, and the normal ETV6 allele was not deleted or rearranged. Although the ABL is known to be involved in various human malignancies, ARG has not been involved in human malignancies despite its high homology to ABL. Thus, this is the first report showing involvement of ARG in human leukemia. The ETV6/ARG protein may be involved in the unique differentiation capacity of this cell line. (C) 2000 by The American Society of Hematology.

AB - The ETV6/TEL gene has been reported to fuse to PDGFRβb MDS1/EV11, BTL, ACS2, STL, JAK2, ABL, CDX2, TRKC, AML1, and MN1. Among them, PDGFRβ, ABL, JAK2, and TRKC are tyrosine kinases (TK). We identified a novel ETV6 partner gene, ARG (ABL-related gene or ABL2), another TK gene in a cell line established from a patient with acute myelogenous leukemia (AML-M3) with a t(15;17)(q22;q11.2) and a t(1;12)(q25;p13), which has the remarkable feature to differentiate to mature eosinophils in culture with all-trans retinoic acid and cytokines. The ETV6/ARG transcripts consisted of exon 1 to 5 of ETV6 and the 3' portion of ARG starting from exon 1B or exon 2, resulting in an open reading frame for a fusion protein consisting of the entire PNT oligomerization domain of ETV6 and all of the functional domains of ARG including the TK domain. This is the same protein structure as identified in the other ETV6 TK fusion proteins. The reciprocal ARG! ETV6 transcript was not expressed, and the normal ETV6 allele was not deleted or rearranged. Although the ABL is known to be involved in various human malignancies, ARG has not been involved in human malignancies despite its high homology to ABL. Thus, this is the first report showing involvement of ARG in human leukemia. The ETV6/ARG protein may be involved in the unique differentiation capacity of this cell line. (C) 2000 by The American Society of Hematology.

UR - http://www.scopus.com/inward/record.url?scp=17544386390&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=17544386390&partnerID=8YFLogxK

M3 - Article

C2 - 10706884

AN - SCOPUS:17544386390

VL - 95

SP - 2126

EP - 2131

JO - Blood

JF - Blood

SN - 0006-4971

IS - 6

ER -