A new exploration in enhancing the resolutionfor high-performance analysis of mono-sulfate glycosaminoglycan disaccharide isomers on a poly(methyl methacrylate) chip

Yong Zhang, Guichen Ping, Bingmei Zhu, Noritada Kaji, Manabu Tokeshi, Yoshinobu Baba

Research output: Contribution to conferencePaper

Abstract

The glycosaminoglycans (GAGs) labeled with 2-aminoacridone, hydrochloride (AMAC) were found to be prevented from adsorption to the surface of polymehylmethacrylate (PMMA) microchips by dynamic coating microchannel with methyl cellulose (MC). To obtain sufficient resolution of mono-sulfate GAG disaccharide isomers on microchip electrophoresis (MCE), buffer pH, β-cyclodextrin (β-CD), and 1,4-dioxane (DO) were systematically investigated. We found that the application of 1,4-dioxane (DO) dramatically improved the resolution of the analytes. ΔDi-triS, ΔDi-UA2S, ΔDi-4S, and ΔDi-6S were baseline separated within 75 sec. The theoretical plate was in the range of 5×105-1×10 6 m-1.

Original languageEnglish
Pages395-397
Number of pages3
Publication statusPublished - Jan 1 2006
Externally publishedYes
Event10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006 - Tokyo, Japan
Duration: Nov 5 2006Nov 9 2006

Other

Other10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006
CountryJapan
CityTokyo
Period11/5/0611/9/06

Fingerprint

Disaccharides
Polymethyl Methacrylate
Glycosaminoglycans
Polymethyl methacrylates
Isomers
Sulfates
Methylcellulose
Cyclodextrins
Microchannels
Electrophoresis
Cellulose
Buffers
Adsorption
Coatings
1,4-dioxane
2-aminoacridone

All Science Journal Classification (ASJC) codes

  • Chemical Engineering (miscellaneous)
  • Bioengineering

Cite this

Zhang, Y., Ping, G., Zhu, B., Kaji, N., Tokeshi, M., & Baba, Y. (2006). A new exploration in enhancing the resolutionfor high-performance analysis of mono-sulfate glycosaminoglycan disaccharide isomers on a poly(methyl methacrylate) chip. 395-397. Paper presented at 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006, Tokyo, Japan.

A new exploration in enhancing the resolutionfor high-performance analysis of mono-sulfate glycosaminoglycan disaccharide isomers on a poly(methyl methacrylate) chip. / Zhang, Yong; Ping, Guichen; Zhu, Bingmei; Kaji, Noritada; Tokeshi, Manabu; Baba, Yoshinobu.

2006. 395-397 Paper presented at 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006, Tokyo, Japan.

Research output: Contribution to conferencePaper

Zhang, Y, Ping, G, Zhu, B, Kaji, N, Tokeshi, M & Baba, Y 2006, 'A new exploration in enhancing the resolutionfor high-performance analysis of mono-sulfate glycosaminoglycan disaccharide isomers on a poly(methyl methacrylate) chip', Paper presented at 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006, Tokyo, Japan, 11/5/06 - 11/9/06 pp. 395-397.
Zhang Y, Ping G, Zhu B, Kaji N, Tokeshi M, Baba Y. A new exploration in enhancing the resolutionfor high-performance analysis of mono-sulfate glycosaminoglycan disaccharide isomers on a poly(methyl methacrylate) chip. 2006. Paper presented at 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006, Tokyo, Japan.
Zhang, Yong ; Ping, Guichen ; Zhu, Bingmei ; Kaji, Noritada ; Tokeshi, Manabu ; Baba, Yoshinobu. / A new exploration in enhancing the resolutionfor high-performance analysis of mono-sulfate glycosaminoglycan disaccharide isomers on a poly(methyl methacrylate) chip. Paper presented at 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006, Tokyo, Japan.3 p.
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