A new exploration in enhancing the resolutionfor high-performance analysis of mono-sulfate glycosaminoglycan disaccharide isomers on a poly(methyl methacrylate) chip

Yong Zhang, Guichen Ping, Bingmei Zhu, Noritada Kaji, Manabu Tokeshi, Yoshinobu Baba

Research output: Contribution to conferencePaper

Abstract

The glycosaminoglycans (GAGs) labeled with 2-aminoacridone, hydrochloride (AMAC) were found to be prevented from adsorption to the surface of polymehylmethacrylate (PMMA) microchips by dynamic coating microchannel with methyl cellulose (MC). To obtain sufficient resolution of mono-sulfate GAG disaccharide isomers on microchip electrophoresis (MCE), buffer pH, β-cyclodextrin (β-CD), and 1,4-dioxane (DO) were systematically investigated. We found that the application of 1,4-dioxane (DO) dramatically improved the resolution of the analytes. ΔDi-triS, ΔDi-UA2S, ΔDi-4S, and ΔDi-6S were baseline separated within 75 sec. The theoretical plate was in the range of 5×105-1×10 6 m-1.

Original languageEnglish
Pages395-397
Number of pages3
Publication statusPublished - Jan 1 2006
Externally publishedYes
Event10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006 - Tokyo, Japan
Duration: Nov 5 2006Nov 9 2006

Other

Other10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006
CountryJapan
CityTokyo
Period11/5/0611/9/06

All Science Journal Classification (ASJC) codes

  • Chemical Engineering (miscellaneous)
  • Bioengineering

Fingerprint Dive into the research topics of 'A new exploration in enhancing the resolutionfor high-performance analysis of mono-sulfate glycosaminoglycan disaccharide isomers on a poly(methyl methacrylate) chip'. Together they form a unique fingerprint.

  • Cite this

    Zhang, Y., Ping, G., Zhu, B., Kaji, N., Tokeshi, M., & Baba, Y. (2006). A new exploration in enhancing the resolutionfor high-performance analysis of mono-sulfate glycosaminoglycan disaccharide isomers on a poly(methyl methacrylate) chip. 395-397. Paper presented at 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006, Tokyo, Japan.