TY - JOUR
T1 - A new family of LTR-like sequences abundantly expressed in rat tumors
AU - Suzuki, Noriaki
AU - Fujiyoshi, Toshinobu
AU - Maehara, Yoshihiko
AU - Takahashi, Keikichi
AU - Yamamoto, Mikio
AU - Endo, Hidya
N1 - Funding Information:
ACKNOWLEDGEMENTS We thank Dr. Y. Sakaki and M. Ohara for comments on the manuscript and N. Kinoshita and M. Honda for technical assistance. H. Sato and Kureha Institute kindly provided the tumor cell lines. This work was supported by Grants-in-Aid from the Ministry of Education, Science and Culture, Japan.
PY - 1986/12/9
Y1 - 1986/12/9
N2 - We report the identification of two genome DNA fragments containing middle repetitive sequences abundantly expressed in various rat tumors but rarely in normal tissues. These fragments included homologous regions which belonged to a new family of long terminal repeat (LTR) like sequences, designated RAL elements; one displayed the solitary type and the other a provirus structure. The element was transcribed in a strand specific fashion and started from the presumptive cap site within the RAL element. The presumptive polyA addition site within the element was also utilized as evidence of the analysis of a cDNA clone containing the RAL element. This evidence suggested that transcriptional control signals within the element were functioning. Run on assay revealed that expression of the element was regulated at the transcriptional level.
AB - We report the identification of two genome DNA fragments containing middle repetitive sequences abundantly expressed in various rat tumors but rarely in normal tissues. These fragments included homologous regions which belonged to a new family of long terminal repeat (LTR) like sequences, designated RAL elements; one displayed the solitary type and the other a provirus structure. The element was transcribed in a strand specific fashion and started from the presumptive cap site within the RAL element. The presumptive polyA addition site within the element was also utilized as evidence of the analysis of a cDNA clone containing the RAL element. This evidence suggested that transcriptional control signals within the element were functioning. Run on assay revealed that expression of the element was regulated at the transcriptional level.
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U2 - 10.1093/nar/14.23.9271
DO - 10.1093/nar/14.23.9271
M3 - Article
C2 - 2432476
AN - SCOPUS:0022840450
SN - 0305-1048
VL - 14
SP - 9271
EP - 9289
JO - Nucleic Acids Research
JF - Nucleic Acids Research
IS - 23
ER -