A new highly selective and specific anti-puerarin polyclonal antibody for determination of puerarin using a mannich reaction hapten conjugate

Orapin Udomsin, Supaluk Krittanai, Tharita Kitisripanya, Hiroyuki Tanaka, Waraporn Putalun

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Background: Puerarin (PUE) is a phytoestrogen found in Pueraria candollei and Pueraria lobata. These plants are substantial for traditional medicine in various Asian countries. PUE is a key marker that can be found only in the Pueraria species. Objective: To establish the method for determination of PUE content which is required for quality control of pharmaceutical products. Materials and Methods: PUE-cationized bovine serum albumin conjugate was created via Mannich reaction. After the rabbit immunization, the obtain anti-PUE polyclonal antibody (PAb) was used to develop an enzyme-linked immunosorbent assay (ELISA). Results: An anti-PUE PAb possess a great sensitivity and specificity. The cross-reactivity analysis shows no cross-reaction of an established antibody against other substances. In addition, we successfully developed an indirect competitive ELISA (icELISA) for the quantitative analysis of PUE. The result of method validation conforms to acceptance criteria and correlates with high-performance liquid chromatography, the reference method. The icELISA was applied to determine PUE content in Pueraria spp. plant samples and its derived pharmaceutical products. Conclusion: This highly specific immunogen was created from the Mannich reaction. An icELISA can also be applied to other research propose in the further studies. Abbreviations used: PUE: Puerarin; PAb: Polyclonal antibody; ELISA: Enzyme-linked immunosorbent assay; icELISA: Indirect competitive ELISA; cBSA: Cationized bovine serum albumin.

Original languageEnglish
Pages (from-to)S845-S851
JournalPharmacognosy Magazine
Volume13
Issue number52
DOIs
Publication statusPublished - Oct 1 2017

Fingerprint

Haptens
Enzyme-Linked Immunosorbent Assay
Pueraria
Antibodies
Bovine Serum Albumin
puerarin
Phytoestrogens
Cross Reactions
Traditional Medicine
Quality Control
Pharmaceutical Preparations
Immunization
High Pressure Liquid Chromatography
Rabbits
Sensitivity and Specificity

All Science Journal Classification (ASJC) codes

  • Pharmaceutical Science
  • Drug Discovery

Cite this

A new highly selective and specific anti-puerarin polyclonal antibody for determination of puerarin using a mannich reaction hapten conjugate. / Udomsin, Orapin; Krittanai, Supaluk; Kitisripanya, Tharita; Tanaka, Hiroyuki; Putalun, Waraporn.

In: Pharmacognosy Magazine, Vol. 13, No. 52, 01.10.2017, p. S845-S851.

Research output: Contribution to journalArticle

Udomsin, Orapin ; Krittanai, Supaluk ; Kitisripanya, Tharita ; Tanaka, Hiroyuki ; Putalun, Waraporn. / A new highly selective and specific anti-puerarin polyclonal antibody for determination of puerarin using a mannich reaction hapten conjugate. In: Pharmacognosy Magazine. 2017 ; Vol. 13, No. 52. pp. S845-S851.
@article{99c7a252cf824ba69f29975b8d9b553e,
title = "A new highly selective and specific anti-puerarin polyclonal antibody for determination of puerarin using a mannich reaction hapten conjugate",
abstract = "Background: Puerarin (PUE) is a phytoestrogen found in Pueraria candollei and Pueraria lobata. These plants are substantial for traditional medicine in various Asian countries. PUE is a key marker that can be found only in the Pueraria species. Objective: To establish the method for determination of PUE content which is required for quality control of pharmaceutical products. Materials and Methods: PUE-cationized bovine serum albumin conjugate was created via Mannich reaction. After the rabbit immunization, the obtain anti-PUE polyclonal antibody (PAb) was used to develop an enzyme-linked immunosorbent assay (ELISA). Results: An anti-PUE PAb possess a great sensitivity and specificity. The cross-reactivity analysis shows no cross-reaction of an established antibody against other substances. In addition, we successfully developed an indirect competitive ELISA (icELISA) for the quantitative analysis of PUE. The result of method validation conforms to acceptance criteria and correlates with high-performance liquid chromatography, the reference method. The icELISA was applied to determine PUE content in Pueraria spp. plant samples and its derived pharmaceutical products. Conclusion: This highly specific immunogen was created from the Mannich reaction. An icELISA can also be applied to other research propose in the further studies. Abbreviations used: PUE: Puerarin; PAb: Polyclonal antibody; ELISA: Enzyme-linked immunosorbent assay; icELISA: Indirect competitive ELISA; cBSA: Cationized bovine serum albumin.",
author = "Orapin Udomsin and Supaluk Krittanai and Tharita Kitisripanya and Hiroyuki Tanaka and Waraporn Putalun",
year = "2017",
month = "10",
day = "1",
doi = "10.4103/pm.pm_276_17",
language = "English",
volume = "13",
pages = "S845--S851",
journal = "Pharmacognosy Magazine",
issn = "0973-1296",
publisher = "Medknow Publications and Media Pvt. Ltd",
number = "52",

}

TY - JOUR

T1 - A new highly selective and specific anti-puerarin polyclonal antibody for determination of puerarin using a mannich reaction hapten conjugate

AU - Udomsin, Orapin

AU - Krittanai, Supaluk

AU - Kitisripanya, Tharita

AU - Tanaka, Hiroyuki

AU - Putalun, Waraporn

PY - 2017/10/1

Y1 - 2017/10/1

N2 - Background: Puerarin (PUE) is a phytoestrogen found in Pueraria candollei and Pueraria lobata. These plants are substantial for traditional medicine in various Asian countries. PUE is a key marker that can be found only in the Pueraria species. Objective: To establish the method for determination of PUE content which is required for quality control of pharmaceutical products. Materials and Methods: PUE-cationized bovine serum albumin conjugate was created via Mannich reaction. After the rabbit immunization, the obtain anti-PUE polyclonal antibody (PAb) was used to develop an enzyme-linked immunosorbent assay (ELISA). Results: An anti-PUE PAb possess a great sensitivity and specificity. The cross-reactivity analysis shows no cross-reaction of an established antibody against other substances. In addition, we successfully developed an indirect competitive ELISA (icELISA) for the quantitative analysis of PUE. The result of method validation conforms to acceptance criteria and correlates with high-performance liquid chromatography, the reference method. The icELISA was applied to determine PUE content in Pueraria spp. plant samples and its derived pharmaceutical products. Conclusion: This highly specific immunogen was created from the Mannich reaction. An icELISA can also be applied to other research propose in the further studies. Abbreviations used: PUE: Puerarin; PAb: Polyclonal antibody; ELISA: Enzyme-linked immunosorbent assay; icELISA: Indirect competitive ELISA; cBSA: Cationized bovine serum albumin.

AB - Background: Puerarin (PUE) is a phytoestrogen found in Pueraria candollei and Pueraria lobata. These plants are substantial for traditional medicine in various Asian countries. PUE is a key marker that can be found only in the Pueraria species. Objective: To establish the method for determination of PUE content which is required for quality control of pharmaceutical products. Materials and Methods: PUE-cationized bovine serum albumin conjugate was created via Mannich reaction. After the rabbit immunization, the obtain anti-PUE polyclonal antibody (PAb) was used to develop an enzyme-linked immunosorbent assay (ELISA). Results: An anti-PUE PAb possess a great sensitivity and specificity. The cross-reactivity analysis shows no cross-reaction of an established antibody against other substances. In addition, we successfully developed an indirect competitive ELISA (icELISA) for the quantitative analysis of PUE. The result of method validation conforms to acceptance criteria and correlates with high-performance liquid chromatography, the reference method. The icELISA was applied to determine PUE content in Pueraria spp. plant samples and its derived pharmaceutical products. Conclusion: This highly specific immunogen was created from the Mannich reaction. An icELISA can also be applied to other research propose in the further studies. Abbreviations used: PUE: Puerarin; PAb: Polyclonal antibody; ELISA: Enzyme-linked immunosorbent assay; icELISA: Indirect competitive ELISA; cBSA: Cationized bovine serum albumin.

UR - http://www.scopus.com/inward/record.url?scp=85041566230&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85041566230&partnerID=8YFLogxK

U2 - 10.4103/pm.pm_276_17

DO - 10.4103/pm.pm_276_17

M3 - Article

VL - 13

SP - S845-S851

JO - Pharmacognosy Magazine

JF - Pharmacognosy Magazine

SN - 0973-1296

IS - 52

ER -